The knowledge of the structures of full-length DgcR both in its native and activated form and the model of the Michaelis–Menten complex allows to discuss in detail how signal perception (phosphorylation) is coupled to output activation in a prototypic response regulator with enzymatic function. In...
Synchrotron BioSAXS is limited by radiation damage, where free radicals produced by water radiolysis damage proteins, leading to aggregation and rapid degradation of the SAXS signal. By utilizing ultra-short pulses, all X-rays can be scattered before protein aggregation occurs. With an adjusted ...