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Inheritance and clearance of maternal mRNAs are two of the most critical events required for animal early embryonic development. However, the mechanisms regulating this process are still largely unknown. Here, we show that together with maternal mRNAs, C
(F) Flow-FISH analysis of total BM cells recovered from xenotransplanted mice. Left: Human cells (hCD45+) cells distinguished from mouse cells (hCD45−). Right: Flow-FISH of hCD45+ cells recovered from xenotransplants with AAVS1 versus PARN-targeted HSPCs ± RG7834. One representative ...
The genus alphavirus comprises of 29 members that infect a range of animal hosts such as humans, rodents, fish, horse etc. Other members of this genus include many human and animal viruses like Semiliki Forest virus (SFV), Sindbis virus (SINV), Ross River virus (RRV), Western equine ...
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For immunofluorescence, cells were fixed with 4% (vol/vol) formaldehyde in PBS, quenched with 50 mM NH4Cl in PBS, permeabilized with 0.2% Triton X-100 in PBS and blocked with blocking buffer (0.2% fish skin gelatin in PBS). Primary and secondary antibodies were applied in blocking buffer ...
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