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Inflammation and immune activation have repeatedly been suggested as pathogentic factors in irritable bowel syndrome (IBS). The driving force for immune activation in IBS remains unknown. The aim of our study was to find out if the obligate intracellular
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isolation kit. cd8 + t cells were isolated from the spleen of naive mice using an easysep mouse cd8 + t cell isolation kit (stemcell) according to the manufacturer's instructions and were labeled with 1µm carboxyfluorescein succinimidyl ester (cfse) (biolegend). t cells were stimulated ...
If you have ever been to a Perlman concert,you know that getting on stage is no small achievement for him.He was stricken with polio (小儿麻痹症) as a child,and so he has braces (支架) on both legs and walks with the aid of two crutches (双拐). He walks p...
First, ribosomal RNA was removed using the Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, Wisconsin, USA), and the rRNA-free residue was cleaned by ethanol precipitation. Subsequently, the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, MA, USA) was used to...
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