For SEM detection, the constructs were fixed with 2.5% glutaraldehyde and critical-point dried with CO2. The cell morphology were observed by SEM (JSM-6500LV, Jeol, Japan). Quantitative real-time PCR Total RNA was extracted from cultured cell-scaffold complex using Trizol reagent following the ...
The dried lipids were dissolved in 100 μL isopropanol, vortexed for 10 min, and centrifuged at 10,300×g for 10 min at 4 °C. The supernatant was transferred to UPLC-TQMS (ACQUITY UPLC-Xevo TQ-S, Waters Corp., Milford, MA, USA) equipped with BEH Amide 1.7 µM analytical column ...
Fresh figs break easily ,so they are often dried,to make them easier -to keep and carry around.Fresh figs are full of healthy vitamins(维生素) and dried figs have even more good things.So why not feast on figs?Your body will thank you a lot.根据文章内容选择最佳答案。(...
Patterns of genetic differentiation of mitochondrial genomes and nuclear outlier loci both show a clear clustering among island populations (Figs.2and4B). Outlier loci can reflect genomic regions associated with local adaptive differences [42,43]. Strong differentiation at these regions putatively under ...
(Supplementary Fig.1). The assignment of these two characteristic peaks was investigated by comparing BBT or DTBT with their building blocks (Supplementary Figs.2–12). The two spectral signatures were not observed after deleting the two thiophenes of DTBT (7, Supplementary Fig.13a). Thiophene...
All the samples we tested have proven to be suitable for LED-SIM imaging. Structures of these small organisms are clearly illustrated (Figs.2,3and4). All samples for LED-SIM imaging in current paper were under the excitation of 405 nm LED light and 20×/NA0.45 objective lens, which yield...
T1mutants did not exhibit any difference in either pollen tube or root growth elongation compared to WT. Moreover, no significant difference in pollen tube or primary root growth elongation was observed in unsupplemented germination media, indicating the conditional nature of this phenotype (Figs.9...
The combined organic layer was washed with brine solution (200 mL), dried with sodium sulphate and concentrated under vacuo to give a crude residue. The residue was purified by combi flash using 3% MeOH/DCM to give 35 (11 g, 58.6%) as a yellow solid. 1H NMR (400 MHz; DMSO-d6): ...
5G). Particularly, dried patches were able to swell up to a value of 4000% within the first 30 min before achieving equilibrium within 1 h after immersion. The overall findings suggested that MeHA patches were suitable for wound healing applications since they significantly swell in physiological...
The histopathological analysis revealed, in general, a better health condition of caged fish compared to field-captured fish (Figs.2,3).Oncorhynchus mykissthat were either kept in the hatchery or were exposed to the river water for seven weeks showed particularly less karyolysis, necrosis and infl...