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How to Get Rid of Pop-Up Ads on Windows 8. The occasional pop-up ad can be a minor annoyance, but persistent pop-up ads can grow into a nuisance and make the Windows 8 user experience unpleasant. Pop-up ads are a commonly used advertising medium on websi
For rescue experiments, 4 point mutations were introduced to the MRE region during the oligonucleotide synthesis step to abolish the miRNA-mRNA interaction. 4.6. Proliferation Assays After transduction and puromycin selection, 2 × 104 cells per well were seeded on 96-well plate in 100 µL of ...
Briefly, H1 ESCs (WiCell, Madison, WI, USA) were dissociated into smaller clumps (around 100 µm in diameter) using 0.5 mM EDTA in PBS, embedded in Matrigel (Corning, Corning, NY, USA) and plated with 3ml of an mTeSR1 and neural induction medium (NIM) mixture (3:1 ratio). NIM ...
The bmp2b, bmp1a, tll1, and chrd mRNAs were generated from pCS2 + kzbmp2, pCS2 + bmp1a [11], pCS2 + tll1 [52], and pCS2 + chrd-myc [48] by restriction digestion with NotI and AmpliCap SP6 High Yield Message Maker Kit (CellScript, Madison, WI, USA). The synthesized capped...
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