The cytokine growth-related oncogene (GRO/KC; CXCL1) shows strong, rapid upregulation in dorsal root ganglion (DRG) in both nerve injury and inflammatory pain models. We examined the direct effects of GRO/KC on small diameter DRG neurons, which are predominantly nociceptive. Whole cell voltage...
The cytokine growth-related oncogene (GRO/KC; CXCL1) shows strong, rapid upregulation in dorsal root ganglion (DRG) in both nerve injury and inflammatory pain models. We examined the direct effects of GRO/KC on small diameter DRG neurons, which are predominantly nociceptive. Whole cell voltage...
4A). Cell counts showed that 11% of all DRG neurons were Rem2 positive (782/7271 DRG neurons from 5 biological replicates) and Rem2-labeled neurons were a subpopulation of large diameter DRG neurons (mean diameter = 36.2 ± 0.2 μm, n = 782 from 5 biological ...
It appears to be selective in that it targets the group of neurons whose axons are the small diameter C and Adelta nociceptive fibres.Hamann WAbou Sherif SThompson SHall SEuropean journal of pain: EJPHamann W, Abou-Sherif S, Thompson S, Hall S. Pulsed radiofrequency applied to dorsal ...
neurons for effective transport of mitochondria by sEVs during the resolution of inflammatory pain [21]. In this study, the peak size of sEVs was around 100 nm. However, it is worth noting that mitochondria typically have a diameter greater than 200 nm. Interestingly, it was reported that ...
cells exerted considerable force on the 200 nm-nanopillars, leading to bending of the nanopillars to form larger focal adhesions from adjacent focal complexes. Similarly NIH 3T3 fibroblasts cultured on PMMA nanopillars with a diameter of 70 nm formed very small adhesions, which led to a higher...
Figure 2-4 The image of mouse DRG neurons under microscope. A cell with diameter of about 25 μm was recorded through a microelectrode (white arrow). Figure 2-5 Determination of small DRG neurons. (A) Measurement of conduction velocity (CV), first arrow indicates the artifact evoked by a...
[81]. These findings were subsequently confirmed by Doppler et al. using electron microscopy of dermal nerves obtained using skin biopsy from the proximal and distal leg and index finger. The diameter of unmyelinated dermal axons was reduced at all sites compared to healthy participants and in ...
Ten µg of labeled sEVs were intrathecally injected into each naïve mouse, and 6 h later, we collected the spinal cord and L4-L5 DRG tissues. Tissues were stained for markers of neurons (MAP2A, green), astrocytes (GFAP, green), and microglia/macrophages (IBA1, green), while nuclei...
5A) from DRG neurons with an average diameter between 20-30 μm. We measured current voltage (I-V) relationships (FIG. 5B) and observed that treatment with 5bk reduced T-type calcium current amplitudes between −20 mV and +10 mV test potentials (FIG. 5B). At peak current density (...