In innate immune cells, median PLCγ2 expression levels were only slightly elevated in p.P522R-carriers compared to non-carriers (range from + 6 to + 31%) (Fig. 2 BC). To summarize our findings based on cell counts: Cohort I was representative of healthy individuals of this ...
As the stimulated B-cell receptor could not be targeted for antibody stain to identify cells, the following marker combinations were used to identify B-cell subsets: For IgM stimulation: pre-GC B cells, CD20+CD27-IgG-IgA-; unswitched MBCs, CD20+CD27+IgG-IgA-; class-switched MBCs, CD20+C...
Both TKO and DKO reconstituted mice also had altered frequencies of various leukocyte subsets in the thymus, bone marrow and spleen, displayed leukocyte infiltrates and autoimmune pathology in multiple tissues, as well as elevated levels of anti-nuclear autoantibodies. Interestingly, the additional ...
Results revealed increased ANA levels in sick DKO and TKO chimeras compared with healthy WT controls, indicating the presence of elevated autoantibodies to chromatin (dsDNA and histones) (Figure 5c). BOK/BAX/BAK-deficient leukocytes are resistant to diverse apoptotic stimuli Previous studies have ...