Sleeping Beauty, a wide host-range transposon vector for genetic transformation in vertebrates. J. Mol. Biol . 302 :93–102 (2000).Izsvak Z, Ivics Z, Plasterk RH: Sleeping Beauty, a wide host-range transposon vector for genetic transformation in vertebrates. J Mol Biol. 2000, 302: 93-...
pT2-vector, carrying the sleeping beauty transposon, pCMV-SB100x-vector, carrying the sleeping beauty transposase with high enzymatic activity or pCMV-SBD3-vector carrying the mutated sleeping beauty transposase with low enzymatic activity [50] were provided by Dr. Ivics (Max Delbrück Center for...
(2010). Extending the trans- posable payload limit of Sleeping Beauty (SB) using the herpes simplex virus (HSV)/SB amplicon-vector platform. Gene Ther. 17, 424-431.De Silva, Suresh, et al. "Extending the transposable payload limit of Sleeping Beauty (SB) using the Herpes Simplex Virus (...
The Sleeping Beauty (SB) transposon system is an efficient non-viral gene transfer tool in mammalian cells, but its broad use has been hampered by uncontrolled transposase gene activity from DNA vectors, posing a risk of genome instability, and by the in
Overview of the Sleeping Beauty (SB) transposon system. a The Gateway cloning of pSB vectors. cDNA is cloned into an Entry vector between the attL1 and attL2 sites. In the presence of the LR clonase, recombination occurs between attL1-attR1 and attL2-attR2 to transfer the insert from ...
The Sleeping Beauty (SB) transposon system has been used for germline transgenesis of the diploid frog, Xenopus tropicalis. Injecting one-cell embryos with plasmid DNA harboring an SB transposon substrate together with mRNA encoding the SB transposase en
The pPC vector was constructed by ligating the CMV-driven "Sleeping Beauty" transposase gene downstream to the Tc1-like transposon inverted repeat (IR) elements and by inserting the RSV promoter (to drive expression of the gene-of-interest) along with a multiple cloning site (MCS), a ...
Virology Development of a bipartite herpes simplex virus/Sleeping Beauty amplicon vector platform| Toward site-directed integration of large transgenons within the human genome UNIVERSITY OF ROCHESTER William J. Bowers de SilvaSureshThe Herpes Simplex Virus type 1 (HSV-1)-derived amplicon, a vector ...
Sleeping BeautymRNA transfectionTranspositionTransgene expressionProducer cell lineThe use of two-component transposon plasmid vector systems, namely, a transposase construct and a donor vector carrying the gene of interest (GOI) can accelerate the development of recombinant cell lines. However, the ...
B. The Sleeping Beauty transposon system: a non-viral vector for gene therapy. Hum. Mol. Genet. 20, R14–R20 (2011). 6. Swierczek, M., Izsvak, Z. & Ivics, Z. The Sleeping Beauty transposon system for clinical applications. Expert. Opin. Biol. Ther. 12, 139–153 (2012). 7. ...