Here we describe the experimentally determined structure of the isolated CDK2-cyclin A-CKS1-p27-SKP1-SKP2 complex at 3.4 global resolution using cryogenic electron microscopy. This structure supports previous analysis in which p27 was found to be structurally dynamic, transitioning from disordered ...
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Skp2 and its cofactor Cks1 are the substrate-targeting subunits of the SCFSkp2-Cks1 (Skp1/Cul1/F-box protein) ubiquitin ligase complex that regulates entry... T Bashir,NV Dorrello,V Amador,... - 《Nature》 被引量: 726发表: 2004年 CKS1B, overexpressed in aggressive disease, regulates...
When screening of Skp2-Cks1 target inhibitors, there were mainly two methods: AlphaScreen43 assay and HTRF17 assay, both were made up of donor beads, receptor beads, and the molecular complex of interaction. They can stimulate the donor beads by excitation light of specific wavelengths, thereby...
摘要: Examines the role of protein complex p45 in the binding of p19 to cyclin A-CDK2 S phase kinase. Difference of cyclin A-CDK2 complexes in normal and transformed cells; Isolation of cDNA clones that encode p19 and p45; Reconstitution of cyclin A-CDK2-p19-p45 complexes....
It has recently been reported that degradation of Skp2 is mediated by ubiquitin ligase APC/CCdh1 (anaphase-promoting complex/cyclosome and its activator Cdh1).40, 41 Cks1 degradation is also mediated by APC/CCdh1.40 As described above, overexpression of Skp2 and Cks1 is frequently observed an...
[2] Schulman BA,Carrano AC,Finnin MS,et al.Insights into SCF ubiquitin ligases from the structure of the Skpl-Skp2 complex.Nature,2000,408(10):381-386. [3] Wang W,Ungermannova D,Jin JP,et al.Negative regulation of SCF-Skp2 ubiquitin ligase by TGF-beta signaling.J Cell Bio,2005,...
Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication. Eukaryotic cells possess overlapping mechanisms to ensure that DNA replication is restricted to the S phase of the cell cycle. The levels of hOrc1p, the la... J Méndez...
SKP1(S-phase kinase-associated protein 1) is also named as EMC19, OCP2, SKP1A, TCEB1L,belongs to the SKP1 family.It is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression,...
The Skp1-Cul1-F-box (SCF) and anaphase- promoting complex (APC) ubiquitin ligases target numerous components of the cell cycle machinery for destruction. Throughout the cell cycle, these ligases cooperate to maintain precise levels of key regulatory proteins, and indirectly, each other. Recently,...