Postfixation, tissues are hybridized with LNA double labeled probes with digoxygenin. Detection of hybridized probes is performed by using an alkaline phosphatase coupled antibody against digoxygenin. The final step involves the use of substrates to develop the color of alkaline phosphatase-LNA-probe...
Thehair follicleis a mini-organ that produces hair. It is composed of papilla, matrix,root sheath, hair fiber, bulge, etc [99]. Hair grows in cycles of three phases: the growth phase (anagen); the regressing phase (catagen); and the quiescent phase (telogen). Thegrowth cyclesare contro...
yourskin also has a setofoil glands, which are properly calledsebaceous glands. Most sebaceous glands wrap around one of the millions of fine hairs that line your body. When your body kicks them into action, they release an oily substance calledsebum, which oozes down the hair follicle and ...
Architecture of the skin and specific display of the interfollicular epidermis.aSkin Composition: the skin epidermis includes the interfollicular epidermis, extending to hair follicle infundibula, and other structures like sebaceous and sweat glands. The dermis, separated from the epidermis by a basement...
Cassettes containing skin were labeled and placed into a container with a fixative [10% (vol/vol) neutral buffered formalin] at 4°C for 16 h. After fixation, skin samples were cut in half and rinsed in Dulbecco's phosphate buffered saline (DPBS) before embedding in paraffin at the Molecul...
NIH 2009R01 AR Roles of Hox genes in skin and hair follicle developmentAwgulewitsch, Alexander / Medical University of South Carolina $286,130 NIH 2008R01 AR Roles of Hox genes in skin and hair follicle developmentAwgulewitsch, Alexander / Medical University of South Carolina $286,130 NIH ...
The skin epidermis is continually influenced by a myriad of internal and external elements. At its basal layer reside epidermal stem cells, which fuels epidermal renovation and hair regeneration with powerful self-renewal ability, as well as keeping dive
(e) Quantification of hair follicles showing SA-b-gal activity and DNA damage foci and in the skin of p53 þ / À and p53TSD/ À mice. Epidermal sheets of p53 þ / À and p53TSD/ À mice were labeled for g-H2A.X (red) as a senescent marker by immunofluorescence (IF) ...
and single-cell RNA-sequencing on genetically labeled myofibroblasts. Using bone marrow transplantation and Cre recombinase-based lineage tracing experiments, we rule out cell fusion events and confirm that hematopoietic lineage cells give rise to a subset of myofibroblasts and rare regenerated adipocytes....
For immunoelectron microscopy, tissue was prepared using a freeze substitution method and immunolabeled using a post-embedding technique (Ishida-Yamamoto et al, 1996). Briefly, tissue was cryoprotected in 15% glycerol in phosphate buffered saline, then rapidly frozen in liquid propane, subjected to ...