Guidance for industry: patient-reported outcome measures: use in medical product development to support labeling claims: draft guidance. Health Qual Life Outcomes. 2006;4:79. Peng JK, Hepgul N, Higginson IJ, Gao W. Symptom prevalence and quality of life of patients with end-stage liver ...
Nonspecific labeling was blocked by use of a mixture of 5% horse serum and 5% fetal calf serum in phosphate-buffered saline solution (PBS). The cells were incubated with anti-desmin antibody (clone 33, 1:300, 1 hour; Dako, Trappes, France) and then with Cy3-conjugated anti-mouse ...
Using 15N stable isotope methodology in arctic ground squirrels (Urocitellus parryii), we provide evidence that free nitrogen is buffered and recycled into essential amino acids, non-essential amino acids and the gamma-glutamyl system during the inter-bout arousal period of hibernation. In the ...
Multiple labeling of SOX2, OCT4, and CDK2 immunofluorescence and NEAT1 FISH further confirmed the colocalization of NEAT1 and SOX2/OCT4/CDK2 during BMSCs aging (Fig. S4E, F). Notably, the predicted interaction domain of SOX2 contains several identified consecutive serine phosphoryla- tion ...
When using the method called LASER Confocal imaging, with immunofluorescence labeling, to identify the specific gene in skeletal muscle satellite cells, the PAX7 expression was positive in cell lines. In addition, PCR amplification bands showed that PAX7, MYF5, and Desmin genes were all clearly ...
The first approach is based on specific labeling of cells by fluorochromes (in case of FACS) or by magnetic nanoparticles coated with antibodies against a particular surface antigen (in the case of magnetic cells sorting, MCS). FACS and MCS allow cell sorting according to their surface marker...
For example, cells may be exposed to a labeling agent for a time equivalent to two doubling times. The mitotic index is the fraction of cells in the culture which have labeled nuclei when grown in the presence of a tracer which only incorporates during S phase (i.e., BrdU) and the ...
Cranial neural crest contributes to the bony skull vault in adult Xenopus laevis: Insights from cell labeling studies As a step toward resolving the developmental origin of the ossified skull in adult anurans, we performed a series of cell labeling and grafting studies of ... JB Gross,J Hanken...
Overall, the spinal cord sections had faint SP labelings in bilateral superficial laminae of dorsal horns in both groups submitted to dry needling, as confirmed by the quantitative analysis, which also showed statistically significant differences between 1D and 5D groups (Scheffe’s method, 1D versu...
Evosep is a liquid chromatography system for improved high-throughput peptide analysis [218]. TIMS uses trapped ion mobility spectrometry with parallel accumulation serial fragmentation (PASEF) operation mode and provides high analytical speed and sensitivity [219]. In addition, multiplexed proteomic ...