The X-ray diffractogram of as-synthesized PtSnCu/C electrocatalyst showed typical face-centered cubic (fcc) structure of Pt alloy and after acid treatment it was observed that the Pt (fcc) structure was preserved. PtSnCu/C electrocatalyst acid-treated was more active for ethanol electro-...
One-h ethanol/distilled water washes were then used to move the animals through 100%, 70%, 50%, 30% ethanol, and then washed twice in tap water. Fish were then macerated using 0.15% trypsin in 65% saturated sodium borax solution at 37°C. Typically fish were digested for 1 to 3 h,...
The cell lysate was mixed with 200 µl of binding buffer (4 M Guanidine HCl, 0.5 M potassium acetate, pH 4.2) and incubated at 70 °C for 10 min, followed by addition of 200 µl of ethanol. The mixture was loaded on a miniprep spin column (epoch bioscience), which was then ...
the right leg of naïve ROSA-NANOG animals was implanted with Dox-Elvax and the left leg with vehicle control DMSO-Elvax. After 2 weeks, the Elvax was removed and TA muscle was harvested 1 week later for evaluation of muscle structure, protein expression and markers of regeneration. We ...
To eliminate contamination, all the reagents used (except for those labeled DNA-free or DNase-free), the laboratory plastic, water, bleach, and ethanol were sterilized (autoclaving) and exposed to UV irradiation for 30 min with BLX-Multichannel BioLink DNA Crosslinker (Vilber) before they were ...
a Functional enrichment of DEGs with the highest FDR values common for WSD/CHOW and CR/CHOW categories. Heat maps of top DEGs involved in ECM organization (b) and cell adhesion (c). Each column represents an individual animal. d Anatomical structure development common gene network indicates up...
structure integrity. Contrary to mammalian fibrosis that persists leading to a consequent reduction of the heart function, the proliferative activity of teleosts cardiomyocytes allows scar replacement and thus heart regeneration [123]. Injury surviving cardiac cells change their gene expression patterns, ...
For these reasons, culturing method in suspension was developed with the objective that the 3D cell–cell interactions would provide a niche-like environment to help maintain cells in a more primitive state [44]. Myosphere corresponds to 3D structure generated from freshly isolated MDCs and that ...
The mammalian skeleton is a weight-bearing structure composed of intricately connected bones that provide a stable internal environment for mineral storage and functional hematopoiesis. Bone is a dynamic hard tissue with a tertiarily-mineralized extracellular matrix and robust self-renewal capacities, under...
Frozen sections were dried at room temperature for 30 min and rehydrated in PBS for 10 min. After staining, sections were dehydrated in 70%, 90%, 100% ethanol for 3 min each and in NeoClear® for 10 min. Slides were mounted using NeoMount® mounting medium (1090160100, VWR...