Full size image Patients with cardiac cachexia exert reduced skeletal muscle OSTN expression To investigate whether or not our findings might also be relevant in patients, we assessed OSTN mRNA levels in vastus lateralis muscle biopsy specimens from control individuals and from patients with sarcopenia...
Scale bar of image with FDB muscle = 500 μm. Scale bar of microscopic fluorescent images = 50 μm. The relationship between pericyte subtypes is unknown. They may represent developmental stages of the same cell. Future studies will use a Cre/loxp system such as NG2–CreER or/and Nestin...
Cross-sectional areas of individual muscle fibers were measured using image analysis, a new and precise technique. Three types of muscle fibers, light, medium and dark, were defined using a modified myosin-ATPase reaction on cryostat-cut sections. Each of the 3 fiber types was found to show ...
Skeletal muscle is an adaptive tissue with the ability to regenerate in response to exercise training. Cross-sectional area (CSA) quantification, as a main parameter to assess muscle regeneration capability, is highly tedious and time-consuming, necessit
Typical two-dimensional (2D) culture models of skeletal muscle-derived cells cannot fully recapitulate the organization and function of living muscle tissues, restricting their usefulness in in-depth physiological studies. The development of functional 3D culture models offers a major opportunity to mimic...
The cross-sectional area (CSA) of the muscle fibers was calculated utilizing ImageJ software (National Institutes of Health, USA). Cell Culture RAW 264.7 cells (leukemia-transformed mouse macrophages, SCSP-5036) and C2C12 cells (murine myoblast cell line, SCSP-505) were obtained from the Shang...
Skeletal muscle has the capacity to adapt to environmental changes and regenerate upon injury. To study these processes, most experimental methods use quantification of parameters obtained from images of immunostained skeletal muscle. Muscle cross-sectio
(a). Scale bar 5 µm. Light microscopic imaging reveals AP-positive areas (red/purple) probably due to presence of activated CD90+fibroblasts (brown) in muscle of ASyS patients (b,c). Scale bar 100 µm. Representative immunofluorescence microscopic images display CD90+fibroblasts in close...
This review comprehensively explores the critical role of calcium as an essential small-molecule biomessenger in skeletal muscle function. Calcium is vital for both regulating muscle excitation–contraction coupling and for the development, maintenance,
1. Gross and Microscopic Anatomy Muscles are surrounded and subdivided by connective tissue sheaths. The epimysium envelops entire muscles. Groups of muscle fibers are arranged in fasciculi that are separated by the perimysium, which is contiguous with the epimysium. The endomysium is a delic...