Skeletal muscle aging is a key contributor to age-related frailty and sarcopenia with substantial implications for global health. Here we profiled 90,902 single cells and 92,259 single nuclei from 17 donors to map the aging process in the adult human int
Representative image of Nestin–GFP + neural cells derived from Nestin–GFP +/NG2–DsRed + cells cultured with cells derived from wild-type FDB muscle co-stained with Tuj1 antibody. Notice that Nestin–GFP −/Tuj1 + cells shown in this image represent neural cells derived from wild-type ...
in heart failure patients, we hypothesized that skeletal muscle as one of the largest tissues of the body might play a pivotal role in preventing the progression of CHF by producing cardioprotective myokines. While it is known that the heart secretes factors under pathological conditions to trigger...
In vivo microscopy of rat skeletal muscle after ischemia using labeled neutrophils (PMN). J Surg Res. 1992;53:563-567.Freischlag JA. In vivo microscopy of rat skeletal muscle after ischemia using labeled neutrophils (PMN). J Surg Res - Richardson, Berker, et al. - 1992...
The molecular mechanisms underlying the sex differences in human muscle morphology and function remain to be elucidated. The sex differences in the skeletal muscle transcriptome in both the resting state and following anabolic stimuli, such as resistance
Also intriguing is the finding that GFP-labeled BMD cells take up residence beneath the basal lamina of skeletal muscle fibers in irradiated transplant recipients following injury (in this case, an exercise model), with subsequent injury provoking an increased contribution of BMD cells to regenerated...
The majority of the calcium required for this process is derived, at least in vertebrate striated muscle fibres, from discrete intracellular stores located at sites within the cell: the terminal cysternae (tc)/junctional SR of the sarcoplasmic reticulum (SR) (Fig. 1 a). These storage sites ...
(B) A section of skeletal muscle (vastus lateralis muscle from rhesus monkey), cut at 90° to the longitudinal axis of the muscle, is shown labeled for laminin (green) and for slow-type myosin (red). Each enclosed ring in the green image represents a separate muscle fiber. The red ...
The experiment was performed using indi- vidual RNA samples (n = 3 ) isolated from different prenatal skeletal muscle specimens. Labeled targets obtained from 5 μ g of total RNA were used for hybridization on each microarray. Purified RNA was labe- led with a miRCURYTM LNA miR...
to the somites, diaphragm and established skeletal muscle in mouse at E11.5 (panel A). In panel B, in situ hybridization of a parasagittal section of a E13.5 (mid-gestation) mouse embryo using a 35S-labeled MyoD riboprobe. Note expression in back, intercostal and limb muscle groups (9)....