International Journal of Molecular Sciences Article IGF-1 Attenuates Hypoxia-Induced Atrophy but Inhibits Myoglobin Expression in C2C12 Skeletal Muscle Myotubes Eva L. Peters 1 ID , Sandra M. van der Linde 1 ID , Ilse S. P. Vogel 1, Mohammad Haroon 1 ,ID Carla Offringa 1, Gerard M. J. ...
For the differentiation experiment, cells were plated at a confluence 5 × 104 cell/cm2 in medium containing 2% Horse Serum (EuroClone) instead of FBS and cultured for 48 h [1]. Bone marrow precursor cells (MONO) were harvested by flushing femurs and tibiae of 2–3 month old WT and ...
When the cells were grown to 80–90% confluence, cell differentiation was induced using a differentiation medium containing DMEM with 2% horse serum (Gibco, Carlsbad, CA, USA). After 1 d of differentiation, the cells were then treated with PST (0.5, 1, or 5 µM) (HPLC purity ≥98%,...
The differentiation medium, containing 2% horse serum (Sigma-Aldrich) as a replacement of FBS in the growth medium, was added 6 h after transfection. The transfection protocol was repeated on day 3 and 5 post-seeding to enhance efficiency of transfection in myotubes. Immunostaining with MF20 ...
Variables Age (years) Height (cm) BMI (kg/m2) Education level Primary Secondary High Physical activity Low Moderate High Meat consumption Poultry Pork Beef Fish Lamb Venison Horseflesh Placebo Mean ± SD (Standard Deviation) 66.4 ± 1.3 162 ± 5.3 26.5 ± 4.4 n% 00 2 22.2 7 77.8 0 4 ...
International Journal of Molecular Sciences Article MEF2A Regulates the MEG3-DIO3 miRNA Mega Cluster-Targeted PP2A Signaling in Bovine Skeletal Myoblast Differentiation Yaning Wang 1,†, Chugang Mei 1,†, Xiaotong Su 1, Hongbao Wang 1,2, Wucai Yang 1,2 and Linsen Zan 1,2,* 1 College...