Ligand binding affinities for ACE-083 were determined by surface plasmon resonance (SPR) using a Biacore T100 instrument (GE Healthcare). Kinetic assays were performed in a capture format at 37 °C as described23, with the following changes: a concentration series of activin A (0.156 nM–10...
Arctiin and arctigenin are natural compounds belonging to the lignin family and are characterized by their dibenzylbutane skeleton [26]. Lignans are plant compounds that have various bioactivities for preventing several diseases such as obesity, cancer and cardiovascular diseases [27,28]. Therefore, ...