Forty microgram of protein was resolved in SDS-PAGE gel and transferred to a PVDF membrane (GE Healthcare UK Ltd., Buckinghamshire, UK) using a Mini Trans-Blot Electrophoretic Transfer Cell device (BioRad). Membranes were blocked with Tris-buffered saline (TBS) containing 0.1% Tween 20 (TBST...
Through The Mental Health Reform Act, various measures would be in place to address the growing Mental Health Care concern. The bill would, “update state funding, improve mental health practices, and increase access to mental health care” (Bowman & Lesniewski). Senator Lamar Alexander and ...
using a Mini Trans-Blot®Electrophoretic Transfer Cell device (BioRad). Membranes were blocked with Tris-buffered saline (TBS) containing 0.1% Tween 20 (TBST) and 5% non-fat dried milk (BD Falcon, Sparks, MD, USA) for 60 min and then incubated overnight with anti-phospho-AMPK (Thr ...