Size-exclusion effects of substrateporous biocatalystimmobilized trypsinbead (spherical) cellulosestructure-activity relationsA model of heterogeneous biocatalysis, in which kinetics and partition effects are connected via the size-exclusion principle, was worked up experimentally and theoretically. The present ...
This might be due to a balance of the solute-gel and solute-solvent interactions.doi:10.1080/00222349208215513D. RadicL. GargalloA. LeónA. HortaTaylor & Francis GroupJournal of Macromolecular Science Part B
The only factors operative here are the effect of changes in the effective size of macromolecules in solution with a change in concentration and the effect of the varying accessible pore volume with varying concentration. The ratio of these two contributions has been investigated both theoretically ...
The main limitation of the SEC method is that it is poorly reproducible in terms of molar mass analysis due to the difficulty to obtain a pure size-exclusion separation for various factors such as concentration effects, osmotic effects, side processes, secondary retention mechanisms, secondary exclus...
Industrial product Marine Collagen Peptide was taken as the research object,the isolation effects of size exclusion chromatography and ion exchange chromatography were studied respectively. 分别探索了分子排阻色谱和离子交换色谱直接梯度洗脱以及先等度洗脱,再梯度洗脱3种洗脱方法的分离效果,发现离子交换色谱先等度...
A. Goyon, A. Beck, J.L. Veuthey, D. Guillarme, S. Fekete, Comprehensive study on the effects of sodium and potassium additives in size exclusion chromatographic separations of protein biopharmaceuticals, J. Pharm. Biomed. Anal. 144 (2017) 242–251. ...
SIZE-EXCLUSION CHROMATHOGRAPHY OF POLY(N-ALKYL ITACONATE)S : NONEXCLUSION EFFECTS The polymer structures have been characterized using NMR spectroscopy and size-exclusion chromatography, and their thermal properties determined using differential ... D Radic F.,L Gargallo G.,A Leon,... 被引量: ...
separations where the peak area of the later eluting analyte of the critical pair is very low in comparison to that of the preceding peak are particularly sensitive to these effects. once a low-dispersion chromatographic system has be...
Results obtained at the same flow velocity on different sized columns will be comparable as far as the effects of flow velocity are concerned. To scale up a separation, follow this advice: Optimize the separation at small scale, with, for example, Superdex™ Increase 10/300 or Hi...
The separation effects of alkylphenol ethoxylates in different liquid chromatography modes,such as reversed-phase liquid chromatography,bonded normal-phase liquid chromatography,silica adsorption chromatography and size exclusion chromatography were investigated. 考察了烷基酚聚氧乙烯醚在反相色谱、正相键合色谱、硅...