To further confirm that microvilli are needed to actively penetrate a glycocalyx barrier and establish close contacts, we used a suite of actin-modifying drugs to alter or ‘paralyze’ J8-GECI microvillus formation (Fig. 2a, b). Using our segmentation and analysis pipeline, we found that the ...
Polarization signals induce mechanical alterations of the nucleus in macrophages As a potent activator of macrophages, lipopolysaccharide (LPS) can induce morphological alterations such as cell spreading, polymerization of cytoskeletal actin filaments, and the formation of filopodia, lamellipodia, and membrane...
[e.g.,arginine vasopressin receptor 2,beta-actin,prohibitin,and heat shock protein family D member 1(HSPD1)]and RNA could be used as functional indicators of male fertility.We sought to clarify the effects of differential mRNA expression of selected genes on several fertilisation parameters,...
52 sigma A4700-.2ML Monoclonal Anti-Actin 0.2ml 3294.14 53 sigma A5602-5MG AMD3100 octahydrochloride hydrate 5mg 1057.68 54 sigma A9528-50MG Amphotericin B solubilized 50mg 30.60 产品留言 标题 联系人 联系电话 内容 验证码 点击换一张 注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息...
To investigate the effects of serum deprivation (SD) on microvesicles (MVs) secreted from human myeloma cells and the implications for disease progression. RPMI 8226, U266, and KM3 human myeloma cells were incubated in medium containing 10% (non-SD) or 1
Cells were fixed with 4% paraformaldehyde for 10 min and stained for actin with 250 ng · ml−1 of phalloidin–tetramethylrhodamine B isothiocyanate (TRITC) and 4′,6-diamidino-2-phenylindole (DAPI) for nuclear staining for 30 min. Cells were mounted onto glass slides with ...
Gene transcript changes were normalized to β-actin levels and relative expression of respective genes was examined in LSK cells from Postn−/− FL tissues as compared with the Postn+/+ FL-derived cells. Unpaired two-tailed Student's t test. n = 4–6. ∗p < 0.0055. (E and F) ...
Abstract Maintenance of skeletal muscle is beneficial in obesity and Type 2 diabetes. Mechanical stimulation can regulate skeletal muscle differentiation, growth and metabolism; however, the molecular mechanosensor remains unknown. Here, we show that SWELL1 (Lrrc8a) functionally encodes a swell-activated...
(pH 7.6), 150 mmol/L NaCl and 0.1% Tween 20] and 5% nonfat dried milk and then incubated with rabbit polyclonal anti-human ZNF224 antibody (Sigma-Aldrich, St Louis, MO, USA) (1:400 in block- ing buffer overnight at 4 °C), followed by rabbit polyclonal anti-human β-actin ...
Female reproduction comes at great expense to energy metabolism compensated by extensive organ adaptations including intestinal size. Upon mating, endocrine signals orchestrate a 30% net increase of absorptive epithelium. Mating increases production of the steroid hormone Ecdysone released by theDrosophilaovar...