respective fractions in the formulations, to determine the absolute size distribution profiles of the LPX nanoparticles formulations, and to derive important size-related parameters such as the size-dependent structure and composition of the particles including the quantification of mRNA molecule per LPX....
Bovine serum albumin (BSA) monomer, BSA dimer, and thyroglobulin (Tg) were used as model proteins. Based on TEM imaging and iSEC, the Nuvia resins have a microgranular structure and large pores (110 nm radius), while the Capto resins have a fibrous structure and smaller pores (32-36 nm...
), the length of the illuminated part of the nanochannel (L), the collection efficiency (c) and the optical properties of the molecule and the nanochannel, defined by the total electric dipole polarizability (αt)24. The αt can be determined by the respective electric dipole polarizabilities ...
The smaller the molecule, the slower they move through the column. Molecules greater than 60,000 pass 15 around the beads and are excluded from the column—also referred to as the exclusion limit of a column. The liquid used to dissolve the biomolecules to make the mobile phase is called ...
Peak broadening can occur on a larger system if system volumes are introduced or if the distribution system of the larger column has lower flow efficiency. This will cause extra dilution of the target molecule or even loss of resolution if the application is sensitive to variations in efficiency...
Further support for a role of β-catenin in postnatal liver growth is revealed in mice with a stable-mutant or full-length β-catenin displaying a three to fourfold or 15% increase in liver size attributed to hepatocyte proliferation, respectively [99,100]. Another molecule which plays an ...
Preparation of protein samples for NMR structure, function, and small-molecule screening studies. Methods in Enzymology. 493, 21-60 (2011). Folta-Stogniew, E., Williams, K. R. Determination of molecular masses of proteins in solution: Implementation of an HPLC size exclusion chromatography ...
Albumin, which is normally protected from leakage by its strong negative charge, demonstrated a clearance pattern similar to that of transferrin, a neutral molecule of similar size. Urinary heparan sulfate excretion was significantly increased in children with DSS. Conclusions. The endothelial size-...
(Calbiochem, San Diego, CA, USA). Briefly, sections were deparaffinized and rehydrated. After incubation with 3% hydrogen peroxide for 5 min at room temperature to block endogenous peroxidase activity, sections were then incubated with 0.2% bovine serum albumin and 0.02% tritonX-100 in PBS ...
For that, we studied two representative label-free biosensors –an immunosensor directed against the milk protein β-LG and a DNA aptasensor directed against the small molecule MC-LR toxin. The immuonsensor is comprised of a polyclonal antibody against β-LG and the aptasensor involves a 60 ...