A Method of Correcting for Flow-Rate Fluctuations in Size Exclusion Chromatography Calculations: Applications to Methylene Chloride/Hexafluoroisopropanol Solvent SystemThe mixture of methylene chloride/hexafluoroisopropanol (70/30, v/v) is an excellent polyester solvent, but its low boiling point causes ...
9 RegisterLog in Sign up with one click: Facebook Twitter Google Share on Facebook size analysis [′sīz ə‚nal·ə·səs] (engineering) particle-size analysis McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc. ...
In size exclusion chromatography the elution volume increases with increasing concentration of injected polymer solutions. Several particular processes contribute to this concentration effect. Under model equilibrium stationary conditions, where the phases remain immobile, it is possible to eliminate dynamic ...
For recovery calculations, all measurements were expressed as percentage of the unfiltered conditioned medium. For the flow cytometry stainings in Fig. 2c, beads were alternatively coated with mouse anti-human CD81 (clone JS-81; BD Biosciences) or mouse anti-human CD9 (clone M-L13; BD ...
Both simple mean field and self-consistent field theory calculations predict size-dependent entropic exclusion. Experimental results obtained with neutral polymer chains grafted onto solid polystyrene latex beads confirm the predictions. 展开 关键词: Chemistry ...
Size-exclusion chromatography (SEC) is widely used in a preparative scale for polishing viruses [14], [15], [16] and VLPs [17], [18] as well as for analytical purposes [19], [20], [21]. The size of enveloped VLPs implies that particles are usually eluted within the void fraction ...
Although A260 and A280 convolution can be mitigated by applying a correction factor to titer calculations, we found that coupling MALS to SEC offers a more direct approach to circumvent this drawback as described below. AAV characterization and titer estimation using size-exclusion chromatography with...
Ion Exchange Chromatography (IEC)Polaritystrong acids, strong bases, weak acids, weak bases, and neutrals Size-exclusion chromatography (SEC) or gel permeation chromatography (GPC)Molecular sizelarge molecular size (LMS), medium molecular size (MMS), and small molecular size (SMS) ...
From proton nuclear magnetic resonance (1 H NMR) (Additional file 1) analysis of the polymer, an estimation of the molecular weight (~9 kDa) was made, which was in agreement with the data obtained from size exclusion chromatography (SEC) (Additional file 2). From the infrared (IR) ...
The strategies include size-selective precipitation39, electrophoresis40,41, and size-exclusion chromatography42,43,44,45, which are based on the size dependence of the solubility of QDs in solution, surface charge, and incorporation into mesopores of the stationary phase, respectively. Unlike binary...