Sequencing (HTS) coupled with metabarcoding analyses allows for a large number of DNA sequences to be amplified directly from environmental samples and these can be used to characterise entire communities34,35,36,37. A recent study used metabarcoding to reconstruct cyanobacterial diversity over the la...
and Ł.P.: performed genome assembly and annotation, assisted in comparative analyses, were responsible for figures preparation and GenBank submissions, drew the cp genome map; A.O. and A.P.: performed DNA extraction, were responsible for genome library preparation and DNA sequencing; K.J.C...
By decomposing a compendia of RNA sequencing (RNA-seq) data with independent component analysis, we obtained 400 independently modulated sets of genes, called iModulons. We (i) performed pan-genome analysis of the phylogroup structure of S. Typhimurium and analyzed the iModulons against this ...
The Invitrogen Easy-DNA gDNA Purification Kit (Thermo Fisher) was used to extract high molecular weight genomic DNA (gDNA) from an overnight culture ofR. toruloidesBOT-A2 grown in YPD medium for long-read sequencing. Quantification of the extracted gDNA was done using a Qubit v3 fluorometer. T...
37、as and sequencing of improvement initiatives With its Process Analysis mindset - The Lean Enterprise, as part of CIP - integrates the strategic Corporate strategies around Process Excellence, Organizational Capability and Organic GrowthHow does adopting The Lean Enterprise principles help the Compan ...
The steps are independent of each other and do not have to all be run. At each step there are also scripts to add the information to an SQL database. The database can also be use to automate the analysis of amplicon sequencing. See theSQL databasesection for more details. ...
Design an implementation road map. Companies should develop a comprehensive, long-term category strategy to incorporate all facilities management trends. A sequencing exercise can help companies to prioritize initiatives. Since technologies will continue to evolve, facilities management leaders shou...
relies on both the gene length and the sequencing read length corrections. However, TPM has been pointed as a flawed normalization method for RNA-Seq normalization between highly different samples56,65, and could be less appropriate for the availableS. mansonidata at different life-cycle stages. ...
Whole-genome sequencing approaches with pooled DNA have recently developed into a powerful tool to systematically identify selection signals at a relatively low cost16, particularly for non-model organisms. In livestock, a pioneering study that used the pooled genome sequencing17reported a number of ...
become much easier since the availability of annotated DNA sequences from the Human Genome Project and cataloged single-nucleotide polymorphisms (SNPs) of major ethnic groups from the HapMap project.1,2 High-throughput of genotyping and sequencing technologies has also facilitated the related research...