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Freeze-dried stems and caps of six cultivated mushroom species, namely Agaricus bisporus (white) Agaricus bisporus (brown), Lentinula edodes, Pholiota nameko, Pleurotus eryngii and Pleurotus ostreatus were subjected to ethanolic extraction. The obtained
(99.99%, purity, Sigma-Aldrich, St. Louis, MO, USA) in stoichiometric amounts. All manipulations were performed under an argon atmosphere. The reaction mixtures were sealed in evacuated quartz ampoules and placed in a programmable furnace. The ampoules then were gradually heated to 900 °C at...
(NRP1-b1), in which vascular endothelial growth factors (VEGFs) bind. In the crystal packing of native human NRP1-b1, the VEGF-binding site is obstructed by a crystallographic symmetry neighbor protein, which prevents the binding of ligands. Six charged amino acids located at the protein ...
The reference compounds utilized in this study were purchased from Cayman Chemicals (Ann Arbor, MI, USA), ChemFaces (Wuhan, Hubei, China), Extrasynthese (Lyon, France), MCE Med Chem Express (Monmouth, NJ, USA), and Sigma-Aldrich (St. Louis, MO, USA), or were isolated and characterized ...