In each of the three main Six clades, the tunicate proteins are basal to the amphioxus and vertebrate proteins, which is contrary to the current view that tunicates are the sister group of the vertebrates (e.g. Blair and Hedges, 2005); this topography is likely due to generally high ...
The three main steps of PCR (denaturation, annealing, and extension) are highly dependent on temperature, so you need a thermal block that has both accurate and uniform temperature from well to well. Otherwise, the reproduc...
In mammals, kidney development proceeds in three successive steps from the initial pronephros via the mesonephros to the adult metanephros. All three stages are characterized by mesenchymal-to-epithelial transformations of cells that are derived from the intermediate mesoderm. It has recently been shown...
In our experience, the dryness of these two steps is essential to a successful radiolabeling as this is a moisture-sensitive reaction. After eluting [18F]F- from the QMA cartridge, a mild phase-transfer reagent (18- crown-6/KHCO3) was added and the [18F]F- was dried at 90 °C for ...
Figure 1. Illustration of the main steps in PCR─denaturation, annealing, extension─to amplify target sequence from a template DNA.Video: Principles of PCR PCR relies on three thermal cycling steps to amplify a target DNA sequence...
(RTBOTA2_003356) can indirectly be connected to nitrogen starvation as it catalyses one of the first steps in the carotenoid pathway, which in general was downregulated during nitrogen starvation in BOT-A2 (Fig.3). This might suggest a redirection of the carbon flux from carotenoids to TAGs....
Base retreated two steps. Snabbis stood frozen solid. Jason looked away. The Clubs all leaned in closer for a better look. Fun Freddie rubbed his hands together in plot, whispering "Oh, boy." Stuffed Eddie quivered. The Muffinman effortlessly made a fist, clenching his fingers into the ...
This was also the only mutant with accumulated levels of trehalose-6-phosphate, indicating that the encoded protein is the main phosphatase under normal conditions. Besides ΔtppA, the most studied deletion mutant in this work was ΔtppB. This gene encodes a protein conserved in filamentous ...
Fast implementation of positron emission tomography (PET) into clinical and preclinical studies highly demands automated synthesis for the preparation of PET radiopharmaceuticals in a safe and reproducible manner. The aim of this study was to develop automated synthesis methods for these six 18F-labeled...
wherein said preparing comprises crossing a plant of the maize variety 8SSIX6027 with a second maize plant; (b) crossing the progeny plant with itself or a second plant to produce a seed of a progeny plant of a subsequent generation; (c) repeating steps (a) and (b) with sufficient inbr...