Figure 1. Illustration of the main steps in PCR─denaturation, annealing, extension─to amplify target sequence from a template DNA.Video: Principles of PCR PCR relies on three thermal cycling steps to amplify a target DNA sequence...
The three main steps of PCR (denaturation, annealing, and extension) are highly dependent on temperature, so you need a thermal block that has both accurate and uniform temperature from well to well. Otherwise, the reprodu...
The strains containing the reporter cassettes were evaluated in two steps. Firstly, five strains per promoter construct were analysed with a BioLector microbioreactor for growth and GFP fluorescence. Secondly, one representative strain from each of the three most promising upregulated and three most pr...
Matsuda et al.: EGF receptor and osteoblastic differentiation Pignoni F, Hu B, Zavitz KH, Xiao J, Garrity PA, Zipursky SL (1997) The eye-specification proteins So and Eya form a complex and regulate multiple steps in Drosophila eye development. Cell 91:881– 891 Sarkar PS, Appukuttan B...
Base retreated two steps. Snabbis stood frozen solid. Jason looked away. The Clubs all leaned in closer for a better look. Fun Freddie rubbed his hands together in plot, whispering "Oh, boy." Stuffed Eddie quivered. The Muffinman effortlessly made a fist, clenching his fingers into the ...
This was also the only mutant with accumulated levels of trehalose-6-phosphate, indicating that the encoded protein is the main phosphatase under normal conditions. Besides ΔtppA, the most studied deletion mutant in this work was ΔtppB. This gene encodes a protein conserved in filamentous ...
groups. We employed the published radiolabeling process but devoted some effort to find the optimal drying condition for [18F]F-, and [18F]F-Gln intermediate . In our experience, the dryness of these two steps is essential to a successful radiolabeling as this is a moisture-sensitive reaction...
3.2. Comparative Analysis of Main Nutritional Metabolites in Six Coarse Cereals To illustrate the nutritional value of different coarse cereals, we compared the accumulation patterns of common nutritional metabolites amino acids, vitamins, carbohydrates, flavonoids, and lipids in six coarse cereals (Figure...
Figure 1. Illustration of the main steps in PCR─denaturation, annealing, extension─to amplify target sequence from a template DNA. Video: Principles of PCR PCR relies on three thermal cycling steps to amplify a target DNA sequ...