The invention provides a way to target RNA editing by adenosine deamination to a chosen adenosine within RNA. An antisense RNA oligonucleotide is used for targeting the entire complex to a specific address on the RNA molecule. A Box B RNA and a λ N-peptide are used as a linkage between ...
Site-directed RNA editing (SDRE) is a general strategy for making targeted base changes in RNA molecules. Although the approach is relatively new, several groups, including our own, have been working on its development. The basic strategy has been to couple the catalytic domain of an adenosine...
Construction of a guide-RNA for site-directed RNA mutagenesis utilising intracellular A-to-I RNA editing. Sci Rep [Internet] 2017; 7:41478. Available from: http://www.nature.com/articles/srep41478%5Cnhttp://www.ncbi.nlm.nih.gov/pubm ed/28148949%5Cnhttp://www.pubmedcentral.nih.gov/...
Site-directed RNA editing is an approach to reprogram genetic information at the RNA level. We recently introduced a novel guideRNA that allows for the recruitment of human ADAR2 to manipulate genetic information. Here, we show that the current guideRNA design is already able to recruit another...
02 February 2017 Construction of a guide-RNA for site-directed RNA mutagenesis utilising intracellular A-to-I RNA editing Masatora Fukuda1, Hiromitsu Umeno1, Kanako Nose1, Azusa Nishitarumizu1, Ryoma Noguchi1 & Hiroyuki Nakagawa2 As an alternative to DNA mutagenesis, RNA mutagenes...
Adenosine deaminases that act on RNA are a conserved family of enzymes that catalyze a natural process of site-directed mutagenesis. Biochemically, they convert adenosine to inosine, a nucleotide that is read as guanosine during translation; thus when editing occurs in mRNAs, codons can be recoded...
RNA editing), RNP formation and intracellular and intercellular transport. We further highlight their roles in transcriptional, post-transcriptional and epigenetic, regulatory processes, such as X chromosome inactivation and genomic imprinting; nuclear subdomain formation; translational control; and modulation...
Li Z, Liu Z-B, Xing A et al (2015) Cas9-Guide RNA directed genome editing in soybean. Plant Physiol 169:960–970. https://doi.org/10.1104/pp.15.00783 Article PubMed PubMed Central CAS Google Scholar Li C, Nguyen V, Liu J et al (2019) Mutagenesis of seed storage protein genes ...
Site-directed RNA editing: recent advances and open challenges. RNA editing by cytosine and adenosine deaminases changes the identity of the edited bases. While cytosines are converted to uracils, adenines are converted... H Khosravi,M Jantsch - 《Rna Biology》 被引量: 0发表: 2021年 Crystal...
As an alternative to DNA mutagenesis, RNA mutagenesis can potentially become a powerful gene-regulation method for fundamental research and applied life sciences. Adenosine-to-inosine (A-to-I) RNA editing alters genetic information at the transcript leve