The invention provides a way to target RNA editing by adenosine deamination to a chosen adenosine within RNA. An antisense RNA oligonucleotide is used for targeting the entire complex to a specific address on the RNA molecule. A Box B RNA and a λ N-peptide are used as a linkage between ...
Site-directed RNA editing allows for the manipulation of RNA and protein function by reprogramming genetic information at the RNA level. For this we assemble artificial RNA-guided editases and demonstrate their transcript repair activity in cells and in developing embryos of the annelid Platynereis du...
Adenosine deaminases that act on RNA are a conserved family of enzymes that catalyze a natural process of site-directed mutagenesis. Biochemically, they convert adenosine to inosine, a nucleotide that is read as guanosine during translation; thus when editing occurs in mRNAs, codons can be recoded...
Construction of a guide-RNA for site-directed RNA mutagenesis utilising intracellular A-to-I RNA editing. Sci Rep [Internet] 2017; 7:41478. Available from: http://www.nature.com/articles/srep41478%5Cnhttp://www.ncbi.nlm.nih.gov/pubm ed/28148949%5Cnhttp://www.pubmedcentral.nih.gov/...
02 February 2017 Construction of a guide-RNA for site-directed RNA mutagenesis utilising intracellular A-to-I RNA editing Masatora Fukuda1, Hiromitsu Umeno1, Kanako Nose1, Azusa Nishitarumizu1, Ryoma Noguchi1 & Hiroyuki Nakagawa2 As an alternative to DNA mutagenesis, RNA mutagenes...
Physical chemistry Site-directed spin labeling studies of the phi29 packaging RNA UNIVERSITY OF SOUTHERN CALIFORNIA Peter Z. Qin YoonBo YoungIn bacteriophage phi29, a protein/RNA complex acts as an ATP driven motor to condense the linear double-stranded DNA genome into a pre-formed protein ...
Our team worked with Tacit Therapeutics to understand the company’s goals and challenges so we could create an emerging biotechnology website customized to fit its needs. The new website highlights the details of Tacit Therapeutics’ next-generation RNA gene therapy and Splicing-Directed RepairTM ...
A TALE nuclease architecture for efficient genome editing. Nat Biotechnol 2011; 29: 143–148. Article CAS Google Scholar Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE et al. RNA-guided human genome engineering via Cas9. Science 2013; 339: 823–826. Article CAS Google ...
www.nature.com/scientificreports OPEN The double-domain cytidine deaminase APOBEC3G is a cellular site-specific RNA editing enzyme received: 15 August 2016 accepted: 16 November 2016 Published: 15 December 2016 Shraddha Sharma, Santosh K. Patnaik, Robert T. Taggart & Bora E. ...
Seamless site-directed mutagenesis is an important technique for studying protein functions, tuning enzyme catalytic activities and modifying genetic elements in multiple rounds because it can insert, delete or substitute nucleotides, DNA segments or even entire genes at the target site without introducing...