1.Site-directed Mutagenesis and Random Mutagenesis of Penicillium Expansum Lipase;扩展青霉脂肪酶的定点突变及随机突变 2.Overexpression and Site-directed Mutagenesis of cry1Aa Gene from Bacillus Thuringiensis苏云金杆菌cry1Aa基因的表达和定点突变 3.Site-Directed Mutagenesis Analyses, Codon Optimization Study ...
codon optimizationmutation efficiencyN‐end rulesynthetic mutationsSite-specific mutagenesis techniques, also known as site-directed mutagenesis (SDM), aim to introduce precise alterations in any coding or noncoding deoxyribonucleic acid (DNA) sequence, usually in vitro. These modifications could be as ...
we performed site-directed mutagenesis studies and cell-based function assays in the presence of the orthosteric agonist NE, with and without ZINC5042. The result indicates that most of the mutations on the residues of the predicted allosteric binding site reduce the role of ZINC5042 in ...
QuikChange Multi Site-Directed Mutagenesis Kit 5 QUIKCHANGE MULTI MUTAGENESIS CONTROL The QuikChange Multi control template and control primer mix are used to test the efficiency of simultaneous site-directed mutagenesis at three independent sites. The 4-kb control template is derived from the pBlue...
Site-directed mutagenesis is used extensively for probing gene function. In this paper we describe an improved megaprimer method to the site-directed mutag... LU Haiqiang,YU Hongwei,R Guo,... - 《Frontiers of Agriculture in China》 被引量: 2发表: 2009年 Site-directed mutagenesis of Aspergill...
Site-directed mutagenesis (SDM) is a key method in molecular biology; allowing to modify DNA sequences at single base pair resolution. Although many SDM methods have been developed, methods that increase efficiency and versatility of this process remain highly desired. Method We present a versatile...
In this study, to improve the substrate affinity of AuMan5A, a glycoside hydrolase (GH) family 5 β-mannanase from Aspergillus usamii, had its directed modification conducted by in silico design, and followed by site-directed mutagenesis. The mutant genes, Auman5A (Y111F) and Auman5A (Y115...
For the negative control, primers spanning an unrelated genomic region within the 3'-UTR of the GAPDH gene were used yielding a 97-bp product. Availability and requirements Project name: Site-specific chromatin immunoprecipitation: A selective method to individually analyze neighboring transcription fact...
Here we evolve the FP-targeting antibody VRC34.01 in vitro to enhance FP-neutralization using site saturation mutagenesis and yeast display. Successive rounds of directed evolution by iterative selection of antibodies for binding to resistant HIV-1 strains establish a variant, VRC34.01_mm28, as a...
In order to confirm the importance of the putative residues involved in substrate binding (Table 1), they were subjected to site-directed mutagenesis and the expressed mutant proteins were affinity-purified before assaying for enzyme activity (Table 2). Regardless of the differences observed in their...