When it comes to achieving effective gene knockdown, Lipofectamine RNAiMAX Transfection Reagent outperforms other siRNA transfection reagents. High knockdown levels of target genes can be achieved with as little as 1 nM siRNA (Figure 1). The Lipofectamine RNAiMAX protocol recommends using 10 nM siRNA...
脂质体RNAiMAX试剂订购信息 货号产品名称规格单价 (CNY)数量 13778100Lipofectamine™ RNAiMAX Transfection Reagent, 0.1 mLEach 1,236.00 13778030Lipofectamine™ RNAiMAX Transfection Reagent, 0.3 mLEach 3,112.00 13778075Lipofectamine™ RNAiMAX Transfection Reagent, 0.75 ...
(final 1.0 nM) to HEK293 cells, leading to more than 80% of "round-up" phenotype of HEK293 cells 48h post transfection over negative control (final 1.0 nM with sham EG5 siRNA) while leading siRNA transfectin reagents, Lipofectamine™ RNAiMAX (RNAiMAX, 1.0 nM EG5 siRNA) / INTERFERin ...
3) siRNA转染试剂的主要种类,脂质体类 非脂质体聚合物类,4) 脂质体,开发较早,主要针对转染质粒DNA等大分子开发,现在也被改良用于siRNA的转染; 适合siRNA与质粒的共转以及一些对毒性要求不高的siRNA转染; 代表产品有invitrogen公司的lipofectamineTM 2000/3000和RNAiMAX,5) 非脂质体聚合物类,为克服脂质体转染试剂的...
Cells were transfected with the corresponding siRNA molecules according to the manufacturer’s protocol (Lipofectamine®RNAiMAX™ Transfection Reagent, ThermoFisher Scientific) and cultured for 24 h at 37 °C. After this, RNA was isolated (PureLink RNA Mini Kit, ThermoFisher Scientific) and ...
I tested DNA/siRNA co-transfection using 293T cells and the results were completely satisfactory. I was able to get 95% knockdown of my target gene at 1.0 nM siRNA as well as expression of plasmid DNA using the recommended protocol. I will have to test and see if other cell lines are ...
Lipofectamine RNAiMAX转染试剂令RNAi技术焕发新生 厂家直销 赛默飞品牌 赛默飞世尔科技(中国)有限公司 查看详情 ￥1.00/支 上海 Polysciences PEI转染试剂26008-5 26008-50 Transporter 5 细胞转染 快递/物流 上海曼博生物医药科技有限公司 2年 查看详情 ￥324.20/盒 北京 Polyplus PEIpro® transfection reagent pei...
The transfection mixture consisted of either Cy5 siRNA containing standard LNPs or 12.5 and 50 μL IL-coated LNPs or 50 μL IL-incorporated LNPs or Cy5 siRNA-Lipofectamine RNAiMAX complexes. Post-transfection, the cells were washed with 200 μL of 1× PBS, gently detached with 100 μL ...
The maximum fold increase in IFIT1 mRNA that was measured was 6-fold induced by Lipofectamine RNAiMAX. Therefore the transfection reagents induce a response in bMDM, but this appears to be quickly dampened down by removing the stimulus. However, using a similar protocol 92 genes were still ...
Polysciences PEI转染试剂26406-1 MAXgene GMP Transfection Reagent 细胞转染 快递/物流 上海曼博生物医药科技有限公司 2年 查看详情 ￥2070.00/个 北京 普利莱 DOTAP真核细胞转染试剂 货号: C1510-0.25 普利莱品牌 北京普利莱基因技术有限公司 4年 查看详情 ￥3623.00/盒 上海 赛默飞life lipo2000转染试剂11668027规...