Single-molecule biophysics articles from across Nature Portfolio Atom RSS Feed Single-molecule biophysics is the study of the dynamics and interactions of individual biomolecules to understand how they carry out their functions in living cells. For example, monitoring the folding properties of single ...
doi:10.1016/B978-0-12-374920-8.00221-6FenzS.F.Comprehensive BiophysicsFenz, S., Pezzarossa, A. & Schmidt, T. The Basics and Potential of Single- Molecule Tracking in Cellular Biophysics, Comprehensive Biophysics Vol 2 (ed. Egelman, Edward H.) (Academic Press, 2012)....
Recent advances in single molecule science have presented a new branch of science: single molecule cellular biophysics, combining classical cell biology with cutting-edge single molecule biophysics. This textbook explains the essential elements of this new discipline, from the state-of-the-art single ...
Many proteins fulfill their biological roles not as monomeric species but as oligomeric assemblies, which often exhibit significant heterogeneity in terms of their degree of oligomerization and relative abundance82,83,84. Oligomeric forms of proteins play important functional roles in cellular physiology bu...
Single-Molecule Biophysics Touch Receptors Introduction The formation of oligomers of single-pass transmembrane (TM) receptors activates numerous fundamental cellular events. Oligomerization is often induced by ligand-binding, and its stabilization involves weak interactions along the whole length of the recep...
Nettels, D., and Schuler, B. (2012) Single-Molecule Fret of Protein-Folding Dynamics. Single-Molecule Biophysics: Experiment and Theory, Vol 146 146, 23-48Komatsuzaki T., Kawakami M., Takahashi S., Yang H., Silbey R. J., Nettels D., Schuler B. (2011) Single-molecule FRET of ...
As anticipated before, the Rayleigh regime represents the lower size limit, that is, when the particle is smaller than the light wavelength and the conditions for Rayleigh scattering are satisfied. Few optical trapping experiments are done in biophysics under this condition, anyway this approach is ...
单分子生物物理学简介(Single molecule biophysics) - 第三章单分子 热度: [2011][Nature]Probing cellular protein complexes using single-molecule pull-down 热度: Recentdevelopmentsin¯uorescenceandforcespectroscopyenableustogobeyondtheensembleaverageandmeasurethebehaviorofindividualbiomacromoleculesThesesingle-molecule...
As molecular and cellular biologists move toward nano-techniques for performing experiments on single molecules rather than on populations of molecules, a comprehensive manual on how (and why) to carry out such experiments is needed. Single-Molecule Techniques: A Laboratory Manual fills this requirement...
Protein–protein interactions (PPIs) are essential for many cellular processes. However, transient PPIs are difficult to measure at high throughput or in complex biological fluids using existing methods. We engineered a genetically encoded sensor for real-time sampling of transient PPIs at single-molec...