Single-cell analysisSystems biologyMass-spectrometrySingle-cell proteomicsIsobaric carrierSample preparationHuman physiology and pathology arise from the coordinated interactions of diverse single cells. However, analyzing single cells has been limited by the low sensitivity and throughput of analytical methods....
Mass spectrometry (MS), the most unbiased technique for single-cell proteomics, facilitates the identification and quantification of thousands of proteins, proteoforms, and post-translational modifications (PTMs) in individual cells. A successful single-cell proteomics workflow hinges on through...
any single-cell proteomics workflow along with high quantitative precision and accuracy. Additionally, the use of TMT multiplexing enables the use of any Orbitrap based mass spectrometer for single-cell proteomics by boosting the signal of the peptides for detection and sequencing by mass ...
Single-cell proteomics can reveal cellular phenotypic heterogeneity and cell-specific functional networks underlying biological processes. Here, we present a streamlined workflow combining microfluidic chips for all-in-one proteomic sample preparation and data-independent acquisition (DIA) mass spectrometry (MS...
Due to extensive optimization efforts, single-cell proteomics by Mass Spectrometry (scp-MS) has emerged as a powerful tool to facilitate proteome profiling from ultra-low amounts of input, although further development is needed to realize its full potential. To this end, we carry out ...
Here, we present a streamlined workflow combining microfluidic chips for all-in-one proteomic sample preparation and data-independent acquisition (DIA) mass spectrometry (MS) for proteomic analysis down to the single-cell level. The proteomics chips enable multiplexed and automated cell isolation/...
developed a SCoPE-MS (Single Cell ProtEomics by Mass Spectrometry) approach for quantitative single-cell analysis (15). We have recently developed a BASIL (Boosting to Amplify Signal with Isobaric Labeling) strategy for enabling comprehensive phosphoproteomic analysis of smaller samples (16) (e.g. ...
To fill this deficiency, we present a comprehensive database, namely Single-cell Proteomic DataBase (SPDB, https:// SPDB ), for general single-cell proteomic data, including antibody-based or mass spectrometry-based single-cell proteomics. Equipped with standardized data process and a user-...
We have developed "Live Single-cell Mass Spectrometry" technique for a direct analysis of endogenous compounds in a single living cell. The technique compr... TS Srinivasan,RR Kannan - Single-Cell Omics 被引量: 0发表: 2019年 Advancing single-cell proteomics and metabolomics with microfluidic tec...
Single-cell proteomics enabled by next-generation sequencing or mass spectrometry Article02 March 2023 Multiplexed single-cell proteomics using SCoPE2 Article29 October 2021 Quantitative single-cell proteomics as a tool to characterize cellular hierarchies ...