Single-cell transcriptome analysis has been revolutionized by DNA barcodes that index cDNA libraries, allowing highly multiplexed analyses to be performed. Furthermore, DNA barcodes are being leveraged for spatial transcriptomes. Although spatial resolut
A Modified Protocol Allows for Library Preparation and Sequencing of Samples from as Low as 100 ng of DNA Input High molecular weight DNA was extracted from a single female mosquito. Given that the genomic DNA had a suitable size range for long-insert PacBio sequencing (Figure 1), the ...