The present invention relates to chimeric particles comprising single stranded RNA (ssRNA), double stranded RNA (dsRNA) and at least one cationic agent, a pharmaceutical composition containing said particles and to a method of producing the same. The particles of the present invention are ...
View chapter Book 2012, Principles and Practice of Pediatric Infectious Diseases (Fourth Edition)Stephanie B. Troy, Yvonne A. Maldonado Related terms: Vaccine Efficacy Vaccination Policy Infection RNA Double Stranded RNA Human Immunodeficiency Virus microRNA View all Topics ...
Single-stranded RNA molecule that can be translated directly, without need for modification. A modification of the DNA amplification method qPCR intended to be applied to RNA. This is accomplished by preceding the qPCR with a RT step that produces a double-stranded DNA copy of the ...
Targeting double-stranded nucleic acids using the λExo–pDNA system λ exonuclease (λExo) binds 5′-phosphorylated single-stranded DNA (pDNA) at complementary regions on double-stranded DNA and DNA–RNA duplexes under ambient conditions without a PAM-like motif. In the presence of Mg2+,λ...
et al. Recognition of double-stranded RNA by human toll-like receptor 3 and downstream receptor signaling requires multimerization and an acidic pH. J Biol Chem. 280, 38133–38145 (2005). Article Google Scholar Smole, U. et al. Differential T-cell responses and allergen uptake after ...
从机制上讲,CTCs的单细胞RNA测序分析显示,在患者和小鼠模型中,有丝分裂基因仅在静止期阶段出现明显的上调,从而实现转移熟练程度。系统地,我们发现关键的昼夜节律激素,如褪黑激素,睾酮和糖皮质激素决定了CTC的产生动力学,因此,胰岛素直接促进肿瘤细胞在体内增殖,但以时间依赖的方式。因此,具有高转移倾向的CTCs的自发...
Most methods for converting RNA into sequencing libraries either do not retain information about which DNA strand was transcribed or required additional steps to mark and destroy or mark and recover one strand of a double-stranded cDNA product. We have performed proof-of-concept experiments wherein...
The invention generally relates to the development of immunogenic compositions comprising a DNA copy of at least one live attenuated plus-sense single-stranded RNA virus genome, such as the genome of a live-attenuated Zika virus (ZIKV), Japanese encephalitis virus (JEV) or yellow fever virus (YF...
Single-cell RNA-Seq was first introduced by Tang et al. [1], using a polyT primer with an anchor sequence to select for the cell’s mRNA. After polyadenylation of the resulting cDNA, a second polyT primer with a different anchor is used to obtain double stranded DNA, which is then PCR...
Molecular-dynamics studies of single-stranded hexitol, altritol, mannitol, and ribose nucleic acids (HNA, MNA, ANA, and RNA, resp.) and of the stability of... " Molecular-Dynamics Studies of Single-Stranded Hexitol, Altritol, Mannitol, and Ribose Nucleic Acids (HNA, MNA, ANA, and RNA, ...