Sequencing libraries prepared from 8 μL of concentrated RNA using the Takara SMARTer Stranded Total RNA-Seq Kit v3—Pico Input Mammalian (634485, Takara) and barcoded using the SMARTer RNA Unique Dual Index Kit (634451, Takara). Library concentration was quantified using a Qubit 3.0 Fluoromete...
CRISPR is an RNA molecule that acts as a guide RNA (gRNA), and when directly introduced into cells together with the second component, the Cas9 enzyme, which can induce double stranded cuts in DNA, effectively edits the DNA. Cas9 is a nuclease derived from a species of streptococcal ...