Single Stranded DNA Binding Proteins are proteins that play crucial roles in DNA replication, recombination, and repair by binding to single-stranded DNA with high affinity, regardless of the DNA sequence. They are present in all life forms and utilize a unique protein fold for DNA binding. ...
Upon entry into eukaryotic cell or as a consequence of replication of the viral genome in the host cell nucleus, the circular genome of ssDNA virus is first converted to a double-stranded form by host DNA polymerase, from which mRNA is then synthesized. Specifically, the viral Rep prote...
To perform single-cell RNA structure probing, we modified RNA in single-stranded regions using structure-probing compounds. We then isolated individual cells, lysed and fragmented the RNAs, enriched for poly(A)+RNAs, and performed reverse transcription (RT) and PCR amplification (Fig.1a). After ...
The structures of the FAAP24 N-terminal and C-ter- minal domains show a typical ERCC4 nuclease domain comprising a central six-stranded β-sheet and five flank- ing α helices on both sides (Figure 1A) and an (HhH)2 domain (Figure 1B), respectively. Therefore, FAAP24 appears to be ...
γ-H2AX staining were used to indicate double-stranded DNA damage and apoptosis [25]. A proprietary image analysis system was used to quantify the number of positive cells over the total number of cells and the average signal emission/nucleus. Western blot...
Cas9 creates a double-stranded DNA break at a predefined site in a target gene, after which endogenous non-homologous end joining repair processes induce a random insertion-deletion (indel) mutation [42,43,44,45]. On average, two thirds of indels in coding regions will induce a frameshift by...
as left-handed DNA does not hybridize with natural right-handed DNA, minimizing interference from cellular DNA during the hybridization between the target and probe. In 2022, Chung et al. [43] developed fluorogenic DNA-PAINT utilizing self-quenching single-stranded probes conjugated with a fluorophor...
The gp32 protein has three domains: the N-terminal B-domain (residues 1–21) responsible for cooperative self-assembly, a core domain (residues 22–253), which contains an OB-fold motif and binds with high affinity to single-stranded DNA, and a C-terminal A-domain (residues 254–301) ...
A recent study174 found that SCP from stranded methane can be produced below 1600 USD/ton. Previous research175 conducted a study on the production of SCP for food from straw, determining a minimum economically viable protein selling price between 5160–9007 €/ton dry mass of protein. Economies...
(PRIMPOL)-mediated repriming of DNA synthesis downstream of lesions, leaving behind single-stranded DNA gaps. Moreover, we find that BRCA2 associates with the essential DNA replication factor MCM10 and this association suppresses PRIMPOL-mediated repriming and ssDNA gap formation, while having no ...