KS M 8447-2012(2023) DNA polymerase I(E.coli)Others: DNA single strand, ssDNA, single stranded dna, single strand dna synthesis, single stranded dna synthesis, single stranded dna synthesis, single-stranded dna purification, dna single strand, amplified single strand dna, single stranded dna ...
Regulation of coliphage f1 single-stranded DNA synthesis by a DNA-binding protein Control of single-strand DNA synthesis in coliphage f1 was studied with the use of mutants which are temperature sensitive in gene 2, a gene essential for ... BJ Mazur,P Model - 《Journal of Molecular Biology...
DNA performs diverse functional roles in biology, nanotechnology and biotechnology, but current methods for autonomously synthesizing arbitrary single-stranded DNA are limited. Here, we introduce the concept of primer exchange reaction (PER) cascades, wh
strand ofDNA synthesismost likely by “organizing” thereplisomevia multiple protein–protein interactions.Bacteriophage T4SSB, gp32, is commercially available and commonly used as an agent for removal of DNAsecondary structurewhen DNAtemplatesprone to secondary structure formation are amplified by PCR, ...
An in vitro single strand DNA synthesizing system has been developed from cells deficient in Kornberg DNA polymerase activity. When X-174 infected cells are gently lysed and sedimented through a sucrose gradient there is a peak of 3H-TTP incorporating activity towards the upper region of the ...
Single-stranded DNA (ssDNA) increases the likelihood of homology directed repair with reduced cellular toxicity. However, ssDNA synthesis strategies are limited by the maximum length attainable, ranging from a few hundred nucleotides for chemical synthesis to a few thousand nucleotides for enzymatic synth...
Related to single-stranded DNA:B DNA DNA A. adenine T. thymine C. cytosine G. guanine DNA (dē′ĕn-ā′) n. A nucleic acid that carries the genetic information in cells and some viruses, consisting of two long chains of nucleotides twisted into a double helix and joined by hydrogen ...
Recognition of single-stranded DNA by the bacteriophage T4-induced type II topoisomerase. The bacteriophage T4-induced type II DNA topoisomerase has been shown previously to make a reversible double strand break in DNA double helices. In addition, this enzyme is shown here to bind tightly and to...
It leads to high yields of a single-stranded DNA of defined length (up to 5000 nt), which is labeled to a high specific activity (1.3 × 10 8 cpm/μg DNA). These hybridization probes are equally sensitive as nick-translated DNA probes, but strand specific. This was tested by slot ...
Intraresidue nuclear Overhauser effect spectroscopy cross peaks were observed at 30 °C and a 200 ms mixing time in the H6H1' region for 5'(dCPCPUPCPCPUPUP) (ssPrODN) but not for 5'(dCCUCCUU) (ssODN), suggesting preorganization of the propynylated single strand. NMR structures of the ...