lighting the fragments to universal adapters; performing strand displacement and extension of the nicked fragments; purifying the double-stranded libation products; capturing the double-stranded libation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fr...
In this way, the end-polishing procedure is expected to make all 3-prime ends mirror what is present at the 5-prime end of the complementary strand. To test these expected differences in DNA termini information, we compared the base composition per position across the start coordinates for ...
Methods for ssDNA-seq were first developed for the sequencing of ancient DNA and typically involve multiple time-consuming steps, including dephosphorylation and denaturation of dsDNA fragments, ligation of a DNA-seq adapter with a primer binding site to the 3′ end of the DNA template strand, is...
The method for constructing a chimeric DNA library of the present invention uses matching regions of heterologous DNA sequences as internal primers and enables highly efficient DNA reassembly between heterologous DNAs having relatively low similarity. Therefore, the inventive method can be effectively use...
DNA strand breaks were evaluated by single cell electrophoresis (comet assay)10. The heart was quickly excised after deep anaesthesia and mounted onto a Langendorff perfusion apparatus41. The hearts were perfused with calcium-free buffer (0-Ca2+buffer: 126 mM NaCl, 8.8 mM KCl, 2.0 mM...
BIOSENSOR FOR DETECTION AND VISUALISATION OF SINGLE-STRANDED DNA A modified protein of the single strand DNA-binding domain, SSB family comprising a detectable label is disclosed. The label has detectable characteristics... M Dillingham,SC Kowalczykowski,M Webb - US 被引量: 0发表: 2011年 Singl...
Index1 (i7) is always sequenced using the bottom strand as template, regardless of the Illumina machine in use. That is why the index sequences are reverse complementary to the primer sequences. IMPORTANT:In a dual-index library, how index2 (i5) is sequenced differs from machines to machine...
The reverse transcriptase is then able to convert from mRNA to the DNA of the TSO and synthesize a cDNA strand using the helper oligonucleotide as a template. The full-length cDNA synthesized by this method contains the complete 5′ end of mRNA concurrently reduces the 3´ bias of ...
single-strandnicksinthelowerstrandpredominateatsitescontainingonlytheleft-sideCSEthatdeterminestheprecisepositionoflowerstrandnicksUpperstrandnickpositionsvarybothinvivoandinvitroA24bpsubstratewasnickedwiththesameprecisionasinlongersubstrates,showingthattheconservedsequencesufficesforprecisenickingbyEndoIIUsingDNAligasein...
For both 3P and 5P experiments, first strand cDNA was generated by incubating the emulsions according to the respective 10x Genomics protocol. Emulsions were then broken and nucleic acids recovered Subsequent library preparation steps are detailed in the section below. Library prep CITE-seq / 3P scR...