Integrating single-cell transcriptomic data across different conditions, technologies, and species. Nat Biotechnol. 2018;36(5):411–20. Article CAS PubMed PubMed Central Google Scholar Stuart T, Butler A, Hoffman P, Hafemeister C, Papalexi E, Mauck WM 3rd, et al. Comprehensive integration ...
ScRNA-seq has the ability to reveal accurate and precise cell types and states. Existing scRNA-seq platforms utilize bead-based technologies uniquely barcoding individual cells, facing practical challenges for precious samples with limited cell number. Here, we present a scRNA-seq platform, named Pair...
EPD210FH and BT214 cells were grown in NeuroCult NS-A Basal Medium (STEMCELL Technologies) supplemented with NeuroCult Proliferation Supplement (STEMCELL Technologies), 2mM L-glutamine 1% Penicillin/Streptomycin, 75ng/ml bovine serum albumin (BSA) and 20ng/ml of EGF (Peprotech) and FGF-basic...
A small amount of RNA (2 ng) was used as input material, and libraries were prepared with a SMART-Seq Stranded Kit according to the user manual (Takara Bio USA, Mountain View, CA, USA). In brief, samples were fragmented at 85 °C for 6 min prior to first-strand synthesis. Illumina ...
The redefined user experience requires higher data rates offered by wireless communication technologies, such as Wi-Fi, long-term evolution (LTE), and fifth-generation new-radio (5G-NR). To achieve the higher data rates in mobile communication devices, a radio frequency (RF) signal(s) may be...
Single-cell sequencing technologies such as snRNA-seq, in combination with the steady development of analytical methods, have greatly advanced our understanding of complex human diseases in the past decade1. These techniques have been employed to study Alzheimer’s disease (AD), a devastating neurodeg...
Clinicians and policy makers should be encouraged to adopt healthcare technologies such as ITCS that will help them to provide quality healthcare despite falling budgets. Abbreviations CI: Confidence interval CMS: Center for Medicare and Medicaid Services ITCS: Integrated twin compression screw PFNA...
(Agilent Technologies, Santa Clara, CA) for 40 h in a rotating 65 °C oven. All microarray slides were scanned using an Agilent 2565C DNA scanner and the images were analyzed with Agilent Feature Extraction version 11.0 using default settings. The aCGH data was assessed with a series of QC...
In contemporary battlefields and target reconnaissance, there is an urgent need for detection technologies under low-illumination, low-visible-light conditions. With the development of low-light complementary metal-oxide-semiconductor sensors, the quality of low-light images has greatly improved. However,...
(This article belongs to the Special Issue Sensing and Computer Vision Technologies in 3D Reconstruction and Understanding)Download keyboard_arrow_down Browse Figures Versions Notes Abstract Three-dimensional human pose estimation from depth maps is a fast-growing research area in computer vision. The...