Single cell protein refers to sources of mixed protein extracted from pure or mixed cultures of algae, yeasts, fungi or bacteria. The production of SCP as a food source (e.g. converting sugar into yeast) has been practiced for millennia. Recently, there has been growing interest in exploring...
°c to maintain protein stability. the ip was conducted using the thermo scientific pierce crosslink magnetic ip/co-ip kit (cat. # 88,805) following the manufacturer’s protocol. initially, the beads were pre-washed with 1x coupling buffer. subsequently, 10 µg of α-syn antibody (211)...
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A key aspect of nearly all single-cell sequencing experiments is dissociation of intact tissues into single-cell suspensions. While many protocols have been optimized for optimal cell yield, they have often overlooked the effects that dissociation can ha
Source data Full size image In this study, although estrous cycle staging was not performed we used a bioinformatic approach to determine the estrous cycle stage of each mouse at the time of euthanasia. To do this we initially integrated our data with single-cell transcriptomic data from estrous...
Chromatin accessibility and protein-DNA interactions Multi-Omics The basic chemistry is very similar, the main differences among those scRNA-seq methods are summarised in the table below. For a detailed discussion, check the text boxes from our review:From Tissues to Cell Types and Back: Single-...
REAGENT or RESOURCESOURCEIDENTIFIER Antibodies PE conjugated Mouse Anti-CD34 (clone 581) BD Biosciences Cat#555822; RRID:AB_396151 PE conjugated Mouse Anti-Human CD36 (clone CB38) BD Biosciences Cat#555455; RRID:AB_395848 FITC conjugated Mouse Anti-Human CD71 (clone YDJ1.2.2) Beckman Coulter...
1,183 (65%) of these contigs showed significant sequence similarity (Blast e < 10-5) with genes within the NCBI non-redundant protein database. An additional 421 contigs were aligned to annotated D. pulex proteins. Therefore, 88.5% of the chosen contigs are given putative functions based on...
CAT-like activities between MNxand natural enzymes (n = 3 independent experiments).dThe energy barriers of MNxin the CAT-mimic reaction simulated by DFT.e,fSchematic illustration of the cyclable catalytic CAT processes for MNx.eFe-Cu-N6andfRh/VN4.gThe CAT processes with MNx(Rh, V, ...
To investigate how AMA neurons relay non-histaminergic signals to the circadian clock, we immunostained the clock protein Timeless (Tim) and pigment dispersing factor (Pdf) to examine whether anterogradetrans-Tango tracing can label clock neurons as their postsynaptic neurons, and found that clock ne...