Surface stain antibodies and live/dead dye (Live/Dead Fixable Yellow Dead Cell Stain Kit, Life Technologies L34968, 0.1 µl/test) were then added, and samples were vortexed and incubated for 30 min at room temperature in the dark. Red blood cells were lysed with 1 ml of 1X ...
Representative examples of the four major patterns of clonal evolution inTP53-sAML patients: bi-allelic mutations (b), hemizygous mutations (c), parallel evolution (d) andJAK2negative bi-allelic evolution (e). The numbers in parenthesis indicate the number of patients in each category...
The tumor microenvironment (TME) in pancreatic ductal adenocarcinoma (PDAC) is a complex ecosystem that drives tumor progression; however, in-depth single cell characterization of the PDAC TME and its role in response to therapy is lacking. Here, we perf
The black line represents the model fit to the data. In the SLD profile (C) X-ray data is shown in solid red line, hydrogenated SB3-18-2 on D2O as blue dashed line and hydrogenated SB3-18-2 on 70% D2O in H2O as green line with crosses. (For interpretation of the references to ...
(B) sm-mRNA-FISH representative image of combination in Figure 5C, showing a single-positive cell (blue, Sall4), double-positive cell (red, Sall4/Fgf4), and triple-positive cell (yellow, Sox2/Sall4/Fgf4). (C–H) Bar plot of the percent of cells with transcripts, quantified by sm-...
White beers owe their name to their straw yellow colour deriving from the use of unmalted wheat, which also supplies a relatively high protein content causing haze formation. This study aimed to develop white-inspired craft beers made with combinations of three mixtures of barley malt/unmalted wh...
POMC-EGFP/AgRP Cre-tdTomato double fluorescence expressing mice were cardiac perfused with 4% PFA and the hypothalamus transversally cut to a thickness of 700 μm using the Leica VT100 S vibratome. For immunostaining, the sliced brain was incubated for 2 h with blocking solution (10% Normal ...
3. Make sure Windows always uses python.exe when you double-clic .py files. (SHIFT+rightclick on a .py file, "Open with...", choose python.exe, check the box "Always use this program...", click Ok) 4. Point your browser at http://localhost/cgi-bin/hello.py You...
To check the linear scaling with m, we take the fit lines at n=60 in the middle of the simulation and we find that doubling m from n to 2n and then 4n increases the time by a factor of 1.9 and then 1.95, slightly less than double and in line with linear scaling. With linear ...
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