A single molecule fluorescence imaging microscope, which was first built based on an inverted microscope was prism type[3]. Epi- and prism type TIRF microscopy were switched by moving a mirror. For TIRF microscopy, a 60° dispersion prism was placed on a quartz slide glass with a gap filled...
We combined single-cell or single-nucleus RNA sequencing for all biopsies with a panel of four spatial expression assays (Slide-seq, MERFISH, ExSeq and CODEX) and H&E staining of consecutive serial sections from up to 15 of these biopsies. We leveraged the coupled measurements to provide ...
Ma, C. et al. Multifunctional T-cell analyses to study response and progression in adoptive cell transfer immunotherapy.Cancer Discov.3, 418–429 (2013).Illustrates the value of single-cell functional proteomics for cancer immunotherapy monitoring in patients. CASPubMedPubMed CentralGoogle Scholar Po...
1. In the case of circular DNAs, it is critical to integrate DNA sequence information with transcriptional output measurements to assess their functional impact on cells. At least three types of circular DNAs can be distinguished in human cells2,3,4,5...
the etiology of cell types along the nascent gut tube. In the latter case, we have added an additional axis of resolution to previous studies by uncovering dorsal–ventral patterning associated with the commitment of cells toward either the esophagus or the lung and trachea. To enable this analy...
Logo Design Course 4 Lessons / 12 Week • 2,193 students • 6 comments
Advanced Text Management Text is essential to most graphical user interfaces, which explains why designers work so much on it. They customize it, translate it, and shape it. Some applications created on TouchGFX Designers can have thousands of text resources, each translated into many languages. ...
In this study, we developed ScSpaMet, a framework for joint protein-metabolite imaging at the single-cell level. The scSpaMet framework allowed systematic single-cell segmentation, phenotyping from protein data, and metabolite profiling on the same tissues at high spatial submicron resolution. While ...
Many essential processes in the cell depend on proteins that use nucleoside triphosphates (NTPs). Methods that directly monitor the often-complex dynamics of these proteins at the single-molecule level have helped to uncover their mechanisms of action. H
In other studies, a single neuron will be extracted from a zipper slice gently placed on a coated glass slide and fixed and strained with antibodies. Two methods for studying mRNA in the same cell will also be compared: in situ hybridization or RT-PCR, in both rats and mice. Another ...