Pseudotime analysis using Monocle 3 alpha, that places the progenitor cell population in the middle of a longer trajectory segment, revealed that pGC (CL15) branched to mural GC (CL11) and mature cumulus GC (CL8 and CL3) (Fig. 6a). As pseudotime analysis is susceptible to be affected by...
If primers with a heterogeneity spacer were used, libraries were sequenced at a concentration of 12 pM, without addition of the PhiX spike-in. High-throughput amplicon sequencing of RNA samples Total RNA was extracted from cells using the RNeasy Mini Kit (Qiagen) and digested with ezDNase ...
Unitary currents through cGMP-dependent channels of retinal rods are observed following incorporation into planar lipid bilayers of native vesicles from purified rod outer segment membranes washed free of soluble and peripheral proteins. The influence of the concentration of cGMP, inhibitors ( cis -...
As a consequence, the methodology allowed the mechanization of procedures to perform standard additions and to construct analytical curves using a single stock standard solution, with very simple and conventional computation of the sample concentration. The method was illustrated with experiments using the...
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Second, we examined the measurement noise of FCS and intracellular variability of concentration. To calculate measurement noise, single-point FCS time series data were divided into five segments, and each segment was separately analyzed to obtain autocorrelation functions and protein concentrations (Fig....
PETE, Pelvic segment transitional epithelium; Mes, mesangial cell; Pod, podocyte. Figure S2. Cell-type deconvolution of the kidney tissues from 9 and 18 weeks post-conception. (a) Spatial location and clustering of ST-seq spots. (b) The proportion of signatures of different scRNA-seq cell ...
Re-activating quiescent adult epicardium represents a potential therapeutic approach for human cardiac regeneration. However, the exact molecular differences between inactive adult and active fetal epicardium are not known. In this study, we combined fet
Single Digit 7 Segment dual-color LED Display, Digit Height: 14.20mm(0.56inch), External Dimensions: 12.60x19.00x8.00mm (L x W x H ) DescriptionPart No.ChipIv(mcd)@20mA Cathode RowAnode RowMaterialColorW LD (nm)One Dot Min.Typ. ...
5d). Flagellar growth of the strain induced with arabinose was faster on average, implying that the pauses that we observed could be partially recovered by increasing the cytoplasmic flagellin concentration. We then repeated single flagellar growth tracking to compare individual flagellar growth under ...