Single-cell technologies, particularly single-cell RNA sequencing (scRNA-seq) methods, together with associated computational tools and the growing availability of public data resources, are transforming drug discovery and development. New opportunities are emerging in target identification owing to improved...
Although single-cell sequencing offers significant advantages in the study of atherosclerosis, there are also limitations and areas that require improvement. Firstly, single-cell sequencing technology still has a certain error rate, particularly in detecting low-expression genes, which can result in false...
Transcription is the primary regulatory step in gene expression. Divergent transcription initiation from promoters and enhancers produces stable RNAs from genes and unstable RNAs from enhancers1,2. Nascent RNA capture and sequencing assays simultaneously
In addition, in our study, smoking was another risk factor for poorer bowel preparation (Fig.3). Other studies also confirm this finding [77,78]. It is assumed that smokers are less likely to follow the instructions before colonoscopy precisely due to lower socioeconomic and health status [79...
Confidence intervals (CIs) were estimated by bootstrapping (sampling with replacement). The 95% CIs were shown as error bars. Single-Cell Data Processing Ct values obtained from the BioMark System were converted into log-based expression values according to a set of rules provided below. Briefly...
1 Today no treatment exists to restore osteoarthritic cartilage, so the treatments are symptom based—pharmacologic or nonpharmacologic—and eventually, surgery may be considered for joint replacement. Among the knee OA treatments, viscosupplementation of the joint with intra-articular (IA) injections ...
Reactions were performed using an Applied Biosystems 7900HT system. qPCR results were analyzed using RQ manager 1.2 software to obtain CT values used for relative quantification to the housekeeping gene Hprt. Confocal imaging of whole cell clusters ISC-enriched cell clusters (ENR + CV) ...
Also discussed are recent developments in parallel single-cell manipulation. Table 1. Single-cell manipulation techniques. 2.1. Fluidic-Based Manipulation 2.1.1. Droplet Microfluidics Droplet microfluidics involves compartmentalizing reactions by isolating molecules, particles or cells into droplets [55]. ...
The main weaker aspect compared to FPs is that labeling is not genetically encoded, so it requires additional steps such as chemical coupling reactions; these can be laborious, require controls of labeling efficiency and lead to aspecific labeling and interactions [27,29,156]. Extensive washing ...
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