Next, coverslips were rinsed three times in 100% ethanol and heat dried in an oven at >90 °C for 30 min. Then, the coverslips were treated with 100 µg ml–1 of poly-d-lysine (Sigma, P6407) in water for a minimum of 1 h at room temperature. Afterwards, coverslips ...
meiotic development, meiotic-to-postmeiotic transition, and round spermatid differentiation; in-depth validation of staging at each time point; and single-cell RNA-seq. To purify homogenous spermatogenic cells, we first engineeredVasa-dTomato
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The chambers were inserted in the oven at 37 °C for 2 h. The chambers were then washed with PBS five times. Next, a solution of \(1.5\,{ \upmu \hbox{l}}\) Alexa647/\(1.5\hbox{ ml}\) was inserted into the chamber and was left there for an hour in room temperature. Finally...