Single-particle (molecule) tracking (SPT/SMT) is a powerful method to study dynamic processes in living bacterial cells at high spatial and temporal resolution. We have performed single-molecule imaging of early DNA double-strand break (DSB) repair events during homologous recombination in the ...
To date, single molecule studies have been reliant on tethering or confinement to achieve long duration and high temporal resolution measurements. Here, we present a 3D single-molecule active real-time tracking method (3D-SMART) which is capable of locking on to single fluorophores in solution ...
3D Single Molecule Tracking in Thick Cellular Samples using Multifocal Plane MicroscopyAurein 2.2Aurein 2.3Oriented circular dichroismSolid state NMRalpha-Helical structureMembrane interactionMembrane compositionAntimicrobial activityOne of the major challenges in single molecule microscopy concerns 3D tracking of ...
摘要: The current models of eukaryotic plasma membrane organization separate the plasma membrane into different environments created by lipids and interactions between membrane proteins and the... 展开 关键词: Fluorophore membrane environment single molecule tracking Brownian motion diffusion lipid domain co...
RNA polymerase, transcription, superresolution, single-molecule tracking, protein-DNA interactionsNational Academy of Sciencesdoi:10.1073/pnas.1507592112Stracy, ... M Stracy,C Lesterlin,GDL Federico,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: ...
These results clearly show that single-molecule tracking can provide novel insights into mobility of proteins in the nucleus that cannot be obtained by conventional fluorescence microscopy. Our results suggest that nuclear processes may not be regulated at the level of physical accessibility but rather ...
Single Molecule Tools, Part B:Super-Resolution, Particle Tracking, Multiparameter, and Force Based MethodsSingle molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to ...
In particular, we focus our discussion on elucidating the plasma membrane organization by single molecule tracking. A diffusing membrane constituent—e.g. a protein or a lipid—experiences a manifold of interactions on its path: the most rapid interactions represent the driving force for free ...
单分子(single-molecule)逻辑电路“在 世界上尚无先例。以色列科学家近日宣布,他们成功研制出一种纳米级 DNA 计算机。 … www.docin.com|基于6个网页 2. 一分子 一分子(Single-Molecule)讯号的特殊感测器。 nanosioe.ee.ntu.edu.tw|基于 1 个网页
Fast 3D Single Molecule Tracking with Multifocal Plane Microscopy in Polarized Epithelia Reveals a Novel Cellular Process of Intercellular Transfer 来自 Semantic Scholar 喜欢 0 阅读量: 4 作者:S Ram,YK Dong,E Ward,R Ober DOI: 10.1016/J.BPJ.2012.11.2962 年份: 2013 ...