Stochastic Optical Reconstruction Microscopy, STORM, is one of a family of Nobel Prize winning super-resolution Single Molecule Localization Microscopies (SMLM) for the visualization of biological systems with an optical resolution measured in the tens of nanometers (nm) in the x, y, and z ...
single moleculesuper-resolution nanoscopySingle-molecule localization microscopy (SMLM) is a relatively new imaging modality, winning the 2014 Nobel Prize in Chemistry, and considered as one of the key super-resolution techniques. SMLM resolution goes beyond the diffraction limit of light microscopy ...
Single-molecule force spectroscopy has emerged as a powerful tool to investigate the forces and motions associated with biological molecules and enzymatic activity. The most common force spectroscopy techniques are optical tweezers, magnetic tweezers and atomic force microscopy. Here we describe these techn...
We review the use of luminescent nanoparticles in super-resolution imaging and single-molecule tracking, and showcase novel approaches to super-resolution imaging that leverage the brightness, stability, and unique optical-switching properties of these n
Single-molecule Fluorescence 1Introduction Since ancient times, humans have being fascinated by light, which is intimately involved with our existence and strongly affects our daily lives. Already in the Ancient Greece, mathematicians and philosophers were interested in light phenomena, just think of Euc...
Single-molecule spectroscopy uncovers information typically obscured by ensemble averaging in other methods. Widely used single-molecule techniques include epifluorescence, confocal and total internal reflection microscopy. Each of these has advantages as well as disadvantages, pertaining specifically to signal...
Arguably some of the most interesting biology exists within structurally heterogeneous datasets, and single-particle cryo-EM is, at its core, a single molecule technique that has a unique capability to make sense of such data. A comprehensive approach is required to make sense of increasingly more...
52Altmetric Metrics Abstract Tremendous progress in nanotechnology has enabled advances in the use of luminescent nanomaterials in imaging, sensing and photonic devices. This translational process relies on controlling the photophysical properties of the building block, that is, single luminescent nanoparticles...
Through molecular engineering, we covalently integrated a single molecule Pd catalyst into nanogapped graphene point electrodes. We detected sequential electrical signals that originated from oxidative addition/ligand exchange, pretransmetallation, transmetallation and reductive elimination in a periodic ...
A higher SNR indicates clearer molecule features and less noise(Kaur and Dong, 2023). PSNR is a widely used metric Single-particle cryo-EM image denoising methods Image denoising is an important step in the processing of single-particle cryo-EM images, aimed at reducing noise and improving the...