Single-molecule localization microscopy (SMLM) is a Nobel Prize winning technique that allows quantitative imaging below the diffraction limit and can be applied to significantly improve our understanding of many biological phenomena. We provide a versatile imaging platform with unrivalled stability and ...
Single-molecule force spectroscopy has emerged as a powerful tool to investigate the forces and motions associated with biological molecules and enzymatic activity. The most common force spectroscopy techniques are optical tweezers, magnetic tweezers and atomic force microscopy. Here we describe these techn...
52Altmetric Metrics Abstract Tremendous progress in nanotechnology has enabled advances in the use of luminescent nanomaterials in imaging, sensing and photonic devices. This translational process relies on controlling the photophysical properties of the building block, that is, single luminescent nanoparticles...
where the experiments are carried out. A motorised holder moves the magnets in the vertical direction, thus tuning the force and inducing a molecule stretch; a magnets rotation can be also realised, which causes
Computer models show two possible forms of the single-molecule, three-wheeled nanoroadster created by scientists at Rice University and the University of Graz. The vehicle runs on a light-powered motor that won its inventor a Nobel Prize this year. Credit: Alex Saywell/Leonhard Grill ...
Single-molecule spectroscopy uncovers information typically obscured by ensemble averaging in other methods. Widely used single-molecule techniques include epifluorescence, confocal and total internal reflection microscopy. Each of these has advantages as well as disadvantages, pertaining specifically to signal...
Arguably some of the most interesting biology exists within structurally heterogeneous datasets, and single-particle cryo-EM is, at its core, a single molecule technique that has a unique capability to make sense of such data. A comprehensive approach is required to make sense of increasingly more...
We review the use of luminescent nanoparticles in super-resolution imaging and single-molecule tracking, and showcase novel approaches to super-resolution imaging that leverage the brightness, stability, and unique optical-switching properties of these n
Through molecular engineering, we covalently integrated a single molecule Pd catalyst into nanogapped graphene point electrodes. We detected sequential electrical signals that originated from oxidative addition/ligand exchange, pretransmetallation, transmetallation and reductive elimination in a periodic ...
A higher SNR indicates clearer molecule features and less noise(Kaur and Dong, 2023). PSNR is a widely used metric Single-particle cryo-EM image denoising methods Image denoising is an important step in the processing of single-particle cryo-EM images, aimed at reducing noise and improving the...