Lipid leaflet asymmetrySolid supported lipid bilayerWe demonstrate that 18 keV X-rays can be used to perform reflectometry on single, phospholipid bio-membranes at the solid鈥搇iquid interface. Two different membrane systems were investigated; a single component, fluid phase membrane of 1,2-dioleoyl...
We report here evidence that fast photoemfs across a single interface of such membranes are specific to the bilayer.FELIX T. HONGDAVID MAUZERALLNature New BiologyHong, F. T., and D. Mauzerall. 1972. Photoemf at a single membrane-solution interface specific to lipid bilayers containing magnesium...
The effects of membrane topography on Dxy are included in the Monte Carlo simulations throughout this manuscript to distinguish between z-motion of the lipid and changes to the local lipid mobility within the bilayer. In comparison to conventional MSD vs. Δt analysis, the calculation of Dxy ...
Ion-specific channels in artificial membranes have been formed by the addition of gramicidin A1, alamethicin2, polyene antibiotics3and some proteins4to the solution surrounding the bilayer lipid membrane. Until now there have been no reports of single-ion channels in unmodified lipid membranes. We h...
The best resolution of membrane structure was obtained with samples prepared by high-pressure freezing, and images suggested that the envelope of ASFV consisted of a single lipid membrane. It was less easy to interpret virus structure in chemically fixed or cryosectioned material, and in the ...
Ion-specific channels in artificial membranes have been formed by the addition of gramicidin A, alamethicin, polyene antibiotics and some proteins to the solution surrounding the bilayer lipid membrane. Until now there have been no reports of single-ion channels in unmodified lipid membranes. We have...
Bayburt TH, Grinkova YV, Sligar SG (2002) Self-assembly of discoidal phospholipid bilayer nanoparticles with membrane scaffold proteins. Nano Lett 2:853–856. https://doi.org/10.1021/nl025623k Article CAS Google Scholar Frauenfeld J, Löving R, Armache JP et al (2016) A saposin-lipoprot...
Methods: After removing and homogenizing the rat brain, the supernatant was separately centrifuged in MSE digitonin, H2O and Na2CO3 and mitochondrial inner membrane vesicles were obtained in MSE solution. L-伪- Phosphatidylcholine (membrane lipid) was extracted from fresh egg yolk. Bilayer lipid ...
Membrane topology of bovine adrenocortical cytochrome P-450(C21): Structural studies by trypsin digestion in vesicle membranes Purified adrenocortical microsomal P-450C21 was incorporated into vesicle membranes composed of phosphatidylcholine, phosphatidylethanolamine, and phosphat... S Kominami,H Tagashira,...
J. Single molecule diffusion of membrane-bound proteins: window into lipid contacts and bilayer dynamics. Biophys. J. 99, 2879–2887 (2010). Article CAS PubMed PubMed Central Google Scholar Ziemba, B. P. & Falke, J. J. Lateral diffusion of peripheral membrane proteins on supported lipid ...