In contrast to microfluidic emulsification, in which droplets are created sequentially and thus their number scales with instrument run time, templated emulsification generates monodispersed droplets in parallel by bulk self-assembly, and, thus, the number of droplets (and cells that can be barcoded) ...
condition because it indicates that there is content that cannot be seen.If the content is legitimately bigger than the available space,consider clipping itwitha ClipRect widget before putting itinthe flex,or using a scrollable container rather than a Flex,like a ListView.The specific RenderFlexin...
Then, use "beer_bbknn.py" in your command line (please modify parameters in beer_bbknn.py):python beer_bbknn.py Finally, load the output of beer_bbknn.py and draw UMAP:umap=read.table('bbknn_umap.txt',sep='\t',header=FALSE) umap=as.matrix(umap) rownames(umap)=rownames(pbmc@...
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in terms of computational runtime (d) and peak memory usage (e) on a 100,000 cell reprogramming dataset49(Supplementary Note2). We split the datasets into ten subsets of increasing size and ran each method ten times on each subset. Boxes cover 25% to 75% quantiles, line indicates median...
Configuration just needs to be set once. If you are happy with default configuration, you may use it as well.Goto TopUsing In line ConfigurationsInline configuration means you can set configurations in std::string but make sure you add all the new line characters etc. This is not recommended...
Following preprocessing, batch effect correction was performed on the condition labels. AML matched diagnosis/relapse Raw FASTQ files were accessed from Stetson et al. [7] with the accession code GSE126068. In this dataset, PBMCs were collected from 5 patients with AML on the onset of ...
Genes co-expressed in a cell type- or condition-specific manner should not be present in the bulk or single-cell aggregates. Additional evidence for condition specificity may be derived from comparison to comparable single cell type networks and should be reported. One aspect of single-cell ...
(C–E) Cells are colored by either (C) cell type, (D) condition, or (E) cell cycle phase. (F) Cell-type prediction in WNT3A± organoid culture using logistic regression classifiers trained on all primary small intestinal fetal cells. UMAP plots show overlaid predicted probability for selec...
state changes from HSCs to MEPs, which was consistent with a previous study revealing that the time cost of differentiation from HSCs to MEPs (38.5 hours on average) was significantly less than that required from HSCs to GMPs (209.5 hours on average) under the same environmental condition63 (...