and northbound network isolation solution is used. In terms of the PMS system, Figure 11-1 shows the typical networking diagram of southbound and northbound network isolation. In Figure 11-1, NEs communicate with the PMS system through the southbound interface. Other devices such as the ...
Interventions Multiple-dose intravenous antibiotic prophylaxis extending over 24 hours following surgery, compared with single-dose intravenous antibiotic. The first-choice drug was cloxacillin (2 g per dose). Clindamycin was used (600 mg per dose) for patients with penicillin allergy. Main Outcomes an...
Our findings, supported by single-cell sequencing data as well as results from IHC and IF, allowed us to propose a mechanistic diagram (Additional file 1: Fig S4) illustrating this process. The presence of TLSs in most tumors is associated with better prognosis and clinical outcome [2, 13,...
Cells were treated with 1 µg ml−1 dox (Sigma-Aldrich, D5207) for 48 h, and single cells with strong mCherry fluorescence were sorted for monoclonal generation. The polyclone 3T3-CRISPRi cell line was generated in a similar way. pHR-SFFV-dCas9-BFP-KRAB (Addgene, 46911) ...
Main Tumor protein 53 (TP53) is the most frequently mutated gene in human cancer, typically occurring as a multihit process with a point mutation in one allele and loss of the other wild-type (WT) allele1,2.TP53mutations are also strongly associated with copy number alterations (CNA) and ...
Cardiac fibrosis after myocardial infarction (MI) has been considered an important part of cardiac pathological remodeling. Immune cells, especially macrophages, are thought to be involved in the process of fibrosis and constitute a niche with fibroblast
However, the largest two contained 2160 and 1570 genes respectively, were very densely connected internally, and strongly anti-correlated with each other (Fig. 4A). These factors strongly suggest that these cells, despite being a clonal line, are heterogenous, falling into (at least) two ...
G Venn diagram of genes detected in both scRNA-seq and snRNA-seq analysis of the three human islet samples, UMI > 20. H Unsupervised clustering of scRNA-seq and snRNA-seq integrated data with Louvain resolution of 0.8. I. Dimensional reduction plot grouped by RNA sequencing type Full ...
We next applied TIGON to a time-series scRNA-seq dataset from an A549 cancer cell line, in which cells were exposed to TGFB1 to induce EMT at the first five time points41. Cells collected at different time points were cultured in vitro with the identical initial cell numbers so that the...
Yellow dotted line demarks the Region 2a/2b border. k–l Heat map (k) and summary diagram (l) showing markers distinguishing aECs, cECs, and pECs. m Quantification of the number of ECs per germarium that express the indicated marker genes. Each dot is a germarium. n = 10, 15,...