How chronic mutational processes and punctuated bursts of DNA damage drive evolution of the cancer genome is poorly understood. Here, we demonstrate a strategy to disentangle and quantify distinct mechanisms underlying genome evolution in single cells, d
preset - variable with the instrument preset when - when to play, audioContext.currentTime or 0 to play now, audioContext.currentTime + 3 to play after 3 seconds pitch - note pitch from 0 to 127, for example 2+12*4 to play D of fourth octave (use MIDI key for drums) duration - no...
We are also grateful to the Génotoul bioinformatics platform (Bioinfo Genotoul, Toulouse Midi-Pyrenées) for providing computing resources. Funding This work was supported by institutional Grants from the Institut National de la Santé et de la Recherche Médicale (INSERM), the Université Toulouse ...
Plasmids (the VL library) were isolated using a Plasmid Midi Kit (QIAGEN, Hilden, Germany). The VL library and the VH fragments were digested with NcoI and HindIII (New England Biolabs), and ligation and electroporation were then performed as described for VL. The library was packaged using...
The phagemid vector pHEN I (obtained from the Medical Research Council, UK) was purified from an overnight TG1 bacterial culture using a QIAGEN Plasmid Midi Kit. Restriction enzyme digestion conditions were as described for the insert, but with six units of enzyme per μg of vector DNA. The...
Sections were incubated with a fluorescein-tagged DNA probe (Enzo Life Sciences, Farmingdale, NY, ENZGEN1146000) and counterstained using the fully automated Ventana BenchMark instrument. Single-cell RNA sequencing For 3′ scRNAseq, matched HPV16− and HPV16+ rafts generated from isogenic NIKS ...
The concentration of single-cell suspension was determined using a Cellometer Auto 2000 instrument (Cellometer) and adjusted to 1000 cells/μl. Approximately 14,000 cells were loaded into a CHROMIUM instrument (10x Genomics, CA, USA) according to the standard protocol of the Chromium single cell ...
Musical Instrument Digital interface, or MIDI, is a communication interface provided on virtually every synthesizer made today. 音乐设备数字接口,或称MIDI,实际上是为目前制造的各种合成器提供的一种通信接口。 18. 36kb Controller has RS-485, MODBUS standard field bus-bar communication interface and ...
Free fully-functional tool now Short learning curve Loop-based music production Live external instrument recording Includes three free digital instruments MIDI editor Video tutorials Hundreds of loops and sounds to choose from Cons No audio restoration tools ...
Device Type: Instrument Description: Particle Synth Lepton single is an arpeggio synthesizer that generates random particle sounds based on the input MIDI note, producing up to 5 voices of 16th-note particles. This synthesizer creates a different random timbre each time, with each particle sound gen...