In pigs, eye color is controlled by a single gene with two alleles. A farmer crossed a homozygous green-eyed pig with a homozygous brown-eyed pig, producing blue-eyed offspring. If the blue-eyed pigs are then mated with each other, what would the percent of their offspring most likely ha...
3.2.2 Single-Gene Disorders By definition, single-gene disorders arise as a result of variation in one or both alleles of a gene on an autosome or sex chromosome or in a mitochondrial gene. There have been many investigations into the overall frequency of single-gene disorders. Many early es...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-s
This mutation appears to be endemic and to have spread in the African gene pool by hybridization with some O. barthii accessions. We showed that interaction between the O. sativa and O. glaberrima domestication alleles of SH3 in heterozygotes induces a 'throwback' seed-shattering phenotype ...
(A) LIGER takes as input two or more datasets, which may come from different individuals, species, or modalities, that share corresponding gene-level features. (B) Integrative nonnegative matrix factorization (Yang and Michailidis, 2016) identifies shared and dataset-specific metagenes across dataset...
2d). Taken together with the E14 cells, these results highlight that in the absence of allele-specific measurements, strand-specific 5mC quantification is averaged across both alleles, potentially obscuring a detailed view of the methylation status of the genome. Finally, we find that these two ...
(Extended Data Fig.5c,d). An example gene,Hsp90ab1, had eight distinct coding SNPs differentiating the two alleles across multiple replicates in RNA-seq and Ribo-ITP. The high similarity of paternal allele ratios in RNA expression and ribosome occupancy was consistently observed for multiple ...
b Cells from panel A projected to a two-dimensional space by Monocle2. Each point corresponds to a single cell and cells are colored according to the inferred pseudotime (blue to red). Monocle2 was run with default parameters on the hallmark gene sets G2M Checkpoint downloaded from MSigDB....
Sequence variants and/or copy number variants (deletions/duplications) within the GOLIM4 gene will be detected with >99% sensitivity. Variants classified as unknown significance (VUS), likely pathogenic, or pathogenic will be reported. Benign and lik...
As seen in other single-cell studies, the great diversity in gene expression at the single-cell level, which may serve as a reservoir for cells to acquire drug resistance, cannot be detected with bulk tissue sequencing. Glioblastoma Glioblastoma multiforme is the most common brain and central ...