In pigs, eye color is controlled by a single gene with two alleles. A farmer crossed a homozygous green-eyed pig with a homozygous brown-eyed pig, producing blue-eyed offspring. If the blue-eyed pigs are then mated with each other, what would the percent of their offspring most likely ha...
Animals were randomly assigned to study groups as they were born, and were excluded from the study if the animal, or its sire or dam, could not be confirmed negative for Mamu-B*08 and -B*17 MHC Class I alleles, which are associated with spontaneous lentiviral control52,53. Group sizes ...
For a disorder to be inherited in the autosomal recessive mode, a person needs to have two copies of the mutated gene. Often a proband with a recessive disorder has unaffected parents, each of which has a copy of the mutant. If both parents are carriers (i.e., they have one copy of...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...
(A) LIGER takes as input two or more datasets, which may come from different individuals, species, or modalities, that share corresponding gene-level features. (B) Integrative nonnegative matrix factorization (Yang and Michailidis, 2016) identifies shared and dataset-specific metagenes across dataset...
As seen in other single-cell studies, the great diversity in gene expression at the single-cell level, which may serve as a reservoir for cells to acquire drug resistance, cannot be detected with bulk tissue sequencing. Glioblastoma Glioblastoma multiforme is the most common brain and central ...
The two alleles of each gene are modeled by separate Poisson-Beta distributions with kinetic parameters that are gene- and allele-specific. These two Poisson-Beta distributions share the same cell size factor [Math Processing Error]ϕc, which affects burst size. The true ASE [Math Processing ...
2d). Taken together with the E14 cells, these results highlight that in the absence of allele-specific measurements, strand-specific 5mC quantification is averaged across both alleles, potentially obscuring a detailed view of the methylation status of the genome. Finally, we find that these two ...
Sequence variants and/or copy number variants (deletions/duplications) within the GOLIM4 gene will be detected with >99% sensitivity. Variants classified as unknown significance (VUS), likely pathogenic, or pathogenic will be reported. Benign and lik...
b Cells from panel A projected to a two-dimensional space by Monocle2. Each point corresponds to a single cell and cells are colored according to the inferred pseudotime (blue to red). Monocle2 was run with default parameters on the hallmark gene sets G2M Checkpoint downloaded from MSigDB....