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Screen positive embryos were mounted in 1.5% low melt agarose in ddH2O at 35 °C then samples were incubated at 25 °C up to 15 min to decrease high activity of caTRPA þ embryos. Then on a Petri dish agarose was removed from larva's tail with two diagonal cuts at either side of...
We were unsuccessful in crystallizing L99A, F102A and L103A, the least stable variants, although many attempts were carried out using automated robotics to screen a large number of crystallization conditions. Conformational instability of these variants might be the cause of difficulty in ...
Both of these samples were extracted using a guani- dinium-based lysis buffer and adsorption to a silica spin column (DNeasy Blood and Tissue Kit, Qia- gen®). Thereafter, they were processed as described for MDA-amplified material, starting from the point of 16S rDNA cloning and ...
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As the elongation speed through poly-GR is significantly slower than a general protein sequence, it may cause trailing ribosomes to collide. That might be the reason why many RQC factors were enriched in the CRISPR screen. To test the hypothesis, we evaluated whether ZNF598 knockdown influenced...
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